青霉素过敏病人嗜碱性粒细胞表面CD63的变化  

Changes in the expressions of CD63 on basophils in patients with penicillin allergy

作　　者：杨静[1] 乔海灵[1] 董子明[2] 

机构地区：[1]郑州大学临床药理研究所,河南郑州450052 [2]郑州大学基础医学院病理生理学教研室,河南郑州450052

出　　处：《中国药理学通报》2008年第7期951-955,共5页Chinese Pharmacological Bulletin

基　　金：河南省杰出青年基金资助项目(No0312002100);河南省自然科学基金资助项目(No0211040100);河南省医学创新人才工程资助项目(No2002114)

摘　　要：目的评价嗜碱性粒细胞表面CD63在诊断青霉素过敏反应中的价值。方法采用流式细胞过敏原刺激试验(FAST)的方法检测43例青霉素过敏病人血中嗜碱性粒细胞在9种抗原刺激后(PG、PV、AMP、AX、6-APA、PHA、PHOA、PHPG、NPG)表面CD63的变化。采用放射免疫吸附试验(RAST)和酶联免疫吸附试验(ELISA)检测8种特异性IgE和IgG抗体。结果43例青霉素过敏病人中有28例为CD63阳性,15例健康对照受试者中有1例阳性。其敏感性为65.12%,特异性为93.33%。CD63和特异性IgE在皮试阳性组的敏感性高于特异性IgG(P<0.05),并且在IgE阳性组中,CD63的阳性率高于特异性IgG(P<0.05)。结论CD63可作为检测青霉素类过敏反应嗜碱性粒细胞特异性激活标志物。Aim To evaluate the value of expressions of CD63 on basophils in diagnosing allergy to penicillins. Methods The expressions of CD63 on basophils activated with 9 kinds of antigens [PG： penicillin G, PV：phenoxomethylpenicillin, AMP：ampicillin, AX ： amoxicilloyl, 6-APA ： 6-aminopenicillanic acid, PHA. phenylacetic acid, PHOA ： phenoxacetic acid, PHPG ： N-（p-hydroxyphenyl） glycine, NPG： N-phenylglycine were detected by FAST （Flow cytometric allergen stimulation test ） in 43 patients with penicillins allergy. Eight types of specific IgE and IgG antibodies to penicillins were determinated by RAST and ELISA, respec-tively. Results The sensitivity and specificity of CD63 used as a marker in patients with penicillins allergy were 65.12% and 93.33 % , respectively. Moreover, the sensitivities of CD63 and specific IgE were higher than those of specific IgG in patients with positive skin test （P〈0.05） , and the sensitivity of CD63 was also greater than that of specific IgG in patients with positive IgE （P〈0.05 ）. Conclusion The detection of CD63 expression by FAST was a particularly useful technique in the diagnosis of patients with penicillins allergy.

关 键 词：过敏反应 青霉素 嗜碱性粒细胞 CD63 IGE IGG 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R392.11[医药卫生—基础医学]