严重烧伤后肠黏膜肌球蛋白轻链磷酸化表达改变及其意义  

Role of MLC phosphorylation in intestinal epithelial barrier dysfunction induced by severe burn injury

作　　者：陈传莉[1] 刘依凌[1] 王裴[1] 孙玮[2] 王凤君[1] 

机构地区：[1]第三军医大学西南医院全军烧伤研究所 [2]第三军医大学基础医学部中心实验室,重庆400038

出　　处：《第三军医大学学报》2008年第15期1434-1437,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(30571923);重庆市自然科学基金(2005BB5045)~~

摘　　要：目的探讨肌球蛋白轻链(myosin light chain,MLC)磷酸化在严重烧伤早期对肠黏膜屏障功能及细胞紧密连接相关蛋白变化的作用。方法健康成年SD大鼠48只,采用随机数字表法分为正常对照组及烧伤后1、2、6、12、24h组,用异硫氰酸荧光素-葡聚糖(FITC-Dextran)检测大鼠肠黏膜通透性,免疫荧光法检测肠黏膜细胞紧密连接相关蛋白ZO-1、F-肌动蛋白(F-actin)及磷酸化型MLC(phosphorylated MLC,p-MLC)的变化。结果烧伤后大鼠肠黏膜通透性明显增加,伤后6h达高峰,为对照组的3倍(P<0.01);烧伤后肠上皮通透性的增加伴随有紧密连接相关蛋白ZO-1的明显重分布、细胞紧密连接损害以及肠上皮细胞p-MLC表达的增加。结论MLC磷酸化可能在严重烧伤后肠黏膜屏障功能紊乱及通透性增加的发生机制中起着重要的调控作用。Objective To determine the role of myosin light chain （MLC） phosphorylation in intestinal epithelial barrier dysfunction induced by severe burn injury. Methods Forty-eight healthy adult Sprague Dawley rats were randomly divided into control group, 1, 2, 6, 12 and 24 h postburn groups. The intestinal permeability of all rats was measured by fluorescein-isothiocyanate-dextran （FITC-D）. The changes of tight junction associated protein ZO-1, F-actin and phosphorylated MLC （PMLC） in intestinal epithelia of the rats were analyzed by immunofluorescence. Results The intestinal permeability of the rats increased significantly after burn injury, and peaked at 6 h 3 times higher than the control rats. The increased intestinal permeability was accompanied by the striking reorganization of the tight junction associated protein ZO-1, the damage of the tight junction and the significant increase of PMLC in rats with burned intestinal epithelia. Conclusion MLC phosphorylation might play a very important role in the regulation of intestinal epithelial barrier dysfunction after severe burn injury.

关 键 词：烧伤 肠黏膜 通透性 肌球蛋白轻链磷酸化 ZO-1 

分 类 号：R322.45[医药卫生—人体解剖和组织胚胎学] R329.25[医药卫生—基础医学]