Long-Evans大鼠骨髓间充质干细胞分离培养及生物学特性研究  

Isolation,cultivation and identification of Long-Evans rat bone mesenchymal stem cells

作　　者：刘东宁[1] 阴正勤[1] 吴楠[1] 王艳华[1] 

机构地区：[1]第三军医大学西南医院全军眼科中心

出　　处：《第三军医大学学报》2008年第15期1441-1443,共3页Journal of Third Military Medical University

基　　金：全军医学科研“十一五”计划科技攻关项目(06G072)~~

摘　　要：目的对Long-Evans大鼠骨髓间充质干细胞（bone mesenchymal stem cells，BMSCs）进行分离培养，并检测其生物学特性。方法采用0．85％NH4Cl分离培养Long-Evans大鼠股骨来源BMSCs，检测生长曲线和生长周期，并进行细胞表面抗原和成骨能力鉴定。结果BMSCs体外培养生长状况良好，具有活跃的增殖能力。第5代细胞生长速度较第3代略减慢。细胞周期显示93．79％细胞处于G0～G1期。表面抗原检测绝大多数细胞阳性表达CD29和CD90，几乎不表达CD45。在体外能够向成骨细胞分化。结论Long-Evans大鼠股骨来源骨髓间充质干细胞经0．85％NH4Cl分离培养后，纯度高，具有良好生物学特性。Objective To isolate and cultivate Long-Evans rat bone mesenchymal stem cells （BMSCs） and identify their biological characteristics. Methods BMSCs were obtained from femurs of Long-Evan rats and dealt with 0.85% NH4Cl. The cell growth curve and cell cycle were measured and surface antigens were detected by flow cytometry. Osteoblast differentiation was studied by Alizarin red staining. Results BMSCs had active proliferative ability. The growth velocity of passage 5 cells was slower than that of passage 3 cells. The cell cycle analysis showed that 93.79% of BMSCs was at G0/G1 phase. BMSCs were positive for CD29 and CD44, but negative for CD45. Alizarin red staining of BMSCs after osteoblast induction was positive. Conclusion Highly purified BMSCs obtained from Long-Evan rats by 0.85% NH4Cl show stable biological properties.

关 键 词：骨髓间充质干细胞 细胞培养 生物学特性 

分 类 号：R329-33[医药卫生—人体解剖和组织胚胎学] R329.25[医药卫生—基础医学]