PAX-5、OCT-2及BOB.1在霍奇金淋巴瘤中的表达及其意义  

Expression and significance of PAX-5,OCT-2 and BOB.1 in Hodgkin lymphoma

作　　者：范严严[1] 章容[1] 郭乔楠[1] 

机构地区：[1]第三军医大学西南医院病理学研究所,重庆400038

出　　处：《第三军医大学学报》2008年第15期1466-1469,共4页Journal of Third Military Medical University

基　　金：第三军医大学西南医院临床创新基金(2006A009)~~

摘　　要：目的探讨PAX-5、OCT-2、BOB.1在霍奇金淋巴瘤中的表达情况及其意义。方法对44例霍奇金淋巴瘤行HE染色和应用免疫组织化学PV-9000法标记CD30、CD15、PAX-5、OCT-2、BOB.1、CD20、CD3,按WHO关于淋巴造血组织肿瘤分类(2001年)进行诊断和组织学分型。结果44例霍奇金淋巴瘤中,结节性淋巴细胞为主型(NLPHL)3例(6.8%),经典型霍奇金淋巴瘤(CHL)41例(93.2%)。HRS细胞有36例(81.8%)表达CD30,27例(61.4%)表达CD15,14例(31.8%)表达CD20,43例(97.7%)表达PAX-5,14例(31.8%)表达OCT-2,7例(15.9%)表达BOB.1,其中NLPHL OCT-2、BOB.1均为阳性(100%),CHL中有30例(73.2%)均不表达OCT-2和BOB.1,7例(17.1%)OCT-2(+)、BOB.1(-),1例(2.4%)OCT-2(-)、BOB.1(+)。所有病例均不表达CD3。结论霍奇金淋巴瘤是一种病因不明,诊断较困难的恶性肿瘤,PAX-5、OCT-2、BOB.1标记与传统的CD30、CD15相结合,有助于霍奇金淋巴瘤的诊断及鉴别诊断。Objective To investigate the expressions and significance of PAX-5, OCT-2 and BOB. 1 in Hodgkin lymphoma （HL）. Methods Immunohistochemical method was used to detect the expressions of CD30, CD15, PAX-5, OCT-2, BOB. 1 , CD20 and CD3 in 44 samples of formalin-fixed, paraffin-embedded tissues of HL. The histological classification was carried out according to the WHO classification for tumors of hematopoietic and lymphoid tissues （2001）. Results In all 44 cases, 3 （6.8%） were nodular lymphocyte predominant Hodgkin lymphoma （NLPHL） and 41 （93.2%） were classical Hodgkin lymphoma （CHL）. In Hodgkin and Reed Sternberg cells, CD30 were expressed in 36 samples （81.8%）, CD15 in 27 （61.4%）, CD20 in 14 （31.8%）, PAX-5 in43 （97.7%）, OCT-2 in 14 （31.8%）, and BOB. 1 in7 （15.9%）. All samples of NLPHL expressed OCT-2 and BOB. 1, while in the samples of CHL, 30 samples （73.2%） expressed both OCT-2 and BOB. 1, 7 （17.1%） expressed only OCT-2, 1 （2.4%） expressed only BOB. 1 and all were negative for CD30. Conclusion Hodgkin lymphoma is a rare neoplasm with uncertain cause and difficult to diagnose. The detection of PAX-5, OCT-2 , BOB. 1 in combination with CD30, CD15 will conduce to the diagnosis and differential diagnosis of Hodgkin lymphoma.

关 键 词：霍奇金淋巴瘤 病理 免疫组化 

分 类 号：R446.8[医药卫生—诊断学] R730.21[医药卫生—临床医学]