雌激素对雌性大鼠颏舌肌肌质网Ca^(2+)-ATPase活性及其基因表达的影响  

Effects of estrogen on sarcoplasmic reticulum Ca^(2+)-ATPase activity and gene expression in genioglossal muscle of female rats

作　　者：宋玮华[1] 刘月华[1] 齐娟[1] 邵校[1] 王飞[1] 

机构地区：[1]上海同济大学口腔医学院口腔正畸科,200072

出　　处：《实用口腔医学杂志》2008年第4期536-539,共4页Journal of Practical Stomatology

基　　金：国家自然科学基金项目(编号:30572070;30471913);上海市科学技术委员会科研计划重点项目(编号:05JC14057)

摘　　要：目的:观察雌激素对雌性大鼠颏舌肌细胞肌质网(sarcoplasmic reticulum,SR)Ca2+-ATPase活性及其基因表达的影响,探索其影响颏舌肌功能的分子生物学机制。方法:将30只成年雌性SD大鼠随机分为对照组(control)、去卵巢组(ovariectomy,OVX)、去卵巢回补激素组(ovariectomy with 17β-estradiol,OVX+E2)。术后6周,处死动物,提取颏舌肌SR。采用定磷法检测颏舌肌SR Ca2+-ATPase活性,荧光定量RT-PCR测定SR Ca2+-ATP酶mRNA表达的变化。结果:OVX组血清雌激素水平及子宫湿重较对照组明显降低(雌二醇P<0.01;子宫湿重P<0.01),而OVX+E2组与对照组相比差别无统计学意义(P>0.05)。OVX组颏舌肌SR Ca2+-ATP酶活性较对照组明显降低(P<0.01);OVX+E2组较OVX组有明显提高(P<0.01)、与对照组相比差别无统计学意义(P>0.05)。OVX组颏舌肌SR Ca2+-ATPase mRNA表达较对照组明显降低(P<0.01);OVX+E2组较OVX组有明显提高(P<0.01)、与正常组相比差别无统计学意义(P>0.05)。结论:成年雌性大鼠雌激素水平的变化可引起颏舌肌SR Ca2+-ATP酶活性及其mRNA表达的变化,雌激素可能通过该途径改变颏舌肌功能.Objective：To investigate the effects of estrogen on sarcoplasmic reticulum （SR） Ca^2＋ -ATPase activity and gene expression in female rats genioglossal muscle （GG） , and explore the possible molecular biology mechanism. Methods：30 male rats were randomly divided into 3 groups：Control group, ovariectomy（OVX） group, ovariectomized animals receiving estradiol replacement （ OVX ＋ E2 ） group. The rats were sacrificed 6 weeks after surgery, and the genioglossus was taken out for analysis. Ca^2＋ -ATPase activity was measured by detecting inorganic phosphorus ion, and SR Ca^2＋ -ATPase （SERCA） mRNA level was observed using fluorescence quantitative reverse transcription-polymerase chain reaction （FQ RT-PCR） method. Results：Compared to the control group, SR Ca^2＋- ATPase activity of OVX group remarkably reduced（ P 〈0.01 ）, and SERCA mRNA level was also significantly lowered relevandy（P 〈0.01 ） ; while in OVX ＋ E2 group, there were no significant changes in SR Ca^2＋ -ATPase activity and SERCA mRNA level（ P 〉 0.05 ）. Conclusion： Serum level of estrogen of adult female rats may influence the function of genioglossal muscle by influencing SR Ca^2 ＋ -ATPase activity and the SERCA mRNA expression.

关 键 词：雌激素 颏舌肌 肌质网 CA^2+-ATP酶 基因表达 

分 类 号：R766[医药卫生—耳鼻咽喉科]