不同深度针刺足三里穴对健康大鼠外周血细胞形态数量及淋巴细胞增殖能力和胸腺指数的影响  

Effects of acupuncture at the Zusanli acupoint in various depths on peripheral blood quantity, lymphocyte proliferation and thymic index in healthy rats

作　　者：田阳春[1] 郑玲[2] 袁英[3] 石玥[1] 

机构地区：[1]北京中医药大学针灸学院,北京市100029 [2]北京医院针灸科,北京市100730 [3]首都医科大学宣武医院康复科,北京市100053

出　　处：《中国组织工程研究与临床康复》2008年第29期5687-5690,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：北京中医药大学校级课题(2004-X52)资助项目~~

摘　　要：目的:有研究表明,针刺足三里穴能够提高衰老机体的免疫系统功能,关于足三里穴适宜的针刺深度及对健康机体影响的研究较少。实验观察不同深度针刺足三里穴对健康大鼠免疫功能的影响。方法:实验于2006-09/10在北京联合大学生物活性物质与功能食品北京市重点实验室完成。实验选用健康清洁级雄性Wistar大鼠24只,按随机数字表法分为3组:空白组、皮下组、肌肉组,每组8只。皮下组将毫针沿15°角斜向上平刺入足三里穴皮下,肌肉组将毫针直刺入足三里穴肌肉层,留针20min,1次/d,共14次,空白组不做针刺处理。测定针刺前后各组大鼠体质量的变化、大鼠胸腺指数、脾脏淋巴细胞增殖能力及外周血细胞计数等变化。结果:针刺作用后,肌肉组大鼠胸腺指数及淋巴细胞增殖能力均较空白组明显升高(P<0.05),皮下组和空白组大鼠胸腺指数及淋巴细胞增殖能力无统计学差异(P>0.05),肌肉组与皮下组大鼠白细胞总数、红细胞计数、血红蛋白含量及血小板总数与空白组比较,差异均不显著(P>0.05)。针刺一二周后,肌肉组与皮下组大鼠体重质量均明显低于空白组(P<0.01)。结论:肌肉层深层针刺足三里穴可提高健康大鼠胸腺指数及淋巴细胞增殖能力,增强大鼠机体免疫能力,同时对于机体外周血细胞无明显影响。AIM： According to previous researches, it is indicated that acupuncture at the Zusanli （ST36） acupoint can improve the immune functions of the aging body. However, there are few studies focusing on the proper depth of acupuncture and its effects on the healthy body. The present study aimed to observe the effects on the immune functions of healthy rats by acupuncture at the Zusanli in various depths. METHODS： Experiments were performed at the Beijing Key Lab of Biologically Active Materials and Functional Food in Beijing Union University from September to October 2006. Totally 24 healthy and clean male Wistar rats were divide into 3 groups with random number table, like a control group, a subcutaneous group and a muscle group, and 8 rats in each group. In the subcutaneous group, the needle was evenly put into the rat at the subcutaneous level, with an angle of 15° between the needle and the skin. In the muscle group, the needle was put into the rats at the muscle level, with an angle of 90° between the needle and the skin. In both groups, the needles were kept inside for 20 minutes, once a day, totally 14 times, But nothing was done on the control group, Changes in body mass, thymic indexes, the reproductive capacity of lymphocytes in the spleens and the count of peripheral blood cells were determined in the rats in each group before and after acupuncture at the Zusanli. RESULTS： In comparison with the control group, the thymic indexes and reproductive capacity of the lymphocytes in the spleens in the muscle group were significantly higher （P 〈 0.05）, while there was no statistic difference between the subcutaneous group and control group （P 〉 0.05）. There was no statistically significant difference in the count of white cells, red cells, hemoglobin and platelets after acupuncture in both experimental groups （P 〉 0.05）. After acupuncturing one and two weeks, body mass of rats in the subcutaneous group and muscle groups was obviously lower than the control group （P 〈 0.01）.

关 键 词：足三里 胸腺指数 淋巴细胞增殖能力 针刺深度 

分 类 号：R557.4[医药卫生—血液循环系统疾病]