5-氟尿嘧啶对Jurkat和EL-4细胞周期解偶联的影响  

Effect of 5-flurouracil on cell cycle uncoupling of Jurkat and EL-4 cell lines

作　　者：武宁[1] 李鹏武[1] 王珍琦[1] 贾晓晶[2] 张萱[1] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物学重点实验室,吉林长春130021 [2]吉林大学第二医院放疗科,吉林长春130041

出　　处：《吉林大学学报（医学版）》2008年第4期547-550,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(20675079)

摘　　要：目的:研究5-氟尿嘧啶(5-FU)不同剂量和给药后不同时间Jurkat和EL-4细胞周期解偶联的变化规律。方法:采用不同剂量(0、0.001、0.010、0.100和1.000mg·L-1)5-FU处理Jurkat和EL-4细胞,给药0、4、8、16、24和48h后分别收集细胞,应用碘化丙啶(PI)荧光标记及流式细胞术(FCM)检测分析细胞倍体变化的剂量-效应和时间-效应规律。结果:不同剂量5-FU给药后16h,Jurkat细胞八倍体细胞百分率在0.001、0.010、0.100和1.000mg·L-1各个剂量组均显著低于0mg·L-1组(P<0.01),无剂量依赖性;EL-4细胞八倍体细胞百分率在0.010和0.100mg·L-1组显著高于0mg·L-1组(P<0.05)。0.100mg·L-15-FU给药后,Jurkat细胞八倍体细胞百分率在8、16和24h显著低于相应对照组(P<0.01),48h显著高于相应对照组(P<0.01);EL-4细胞八倍体细胞百分率在4、8和16h显著高于相应对照组(P<0.05),48h显著低于相应对照组(P<0.01)。结论:5-FU可以诱导EL-4细胞发生细胞周期解偶联,而不能诱导Jurkat细胞发生细胞周期解偶联。Objective To observe the effect of 5-flurouracil （5-FU） with different doses and administration time on cell cycle uncoupling of Jurkat and EL-4 cell lines. Methods The dose- and time-effects of cell cycle were detected by flow cytometry （FCM） following staining of Jurkat and EL-4 cells with propidium iodide （PI） and the changes of polyploid cells were observed after treatment with 5-FU with different doses （0, 0.001, 0.010, 0. 100 and 1. 000 mg· L^-1） and administration time （0, 4, 8, 16, 24 and 48 h） . Results As compared with 0 mg· L^-1 group, the percentages of 8N cells were decreased significantly 16 h after exposure of Jurkat cell line to 5-FU with doses of 0. 001, 0. 010, 0. 100 and 1. 000 mg· L^-1 （P〈0. 001） . After exposure of EL-4 cell line to 5-FU with doses of 0. 010, 0. 100 mg· L^-1, the percentages of 8N cells were increased significantly compared with 0 mg· L^-1 group （P〈0.05）. As compared with control, the percentages of 8N cells were decreased at 8, 16 and 24 h and increased significantly at 48 h after exposure of Jurkat cell line to 5-FU with dose of 0. 100 mg· L^-1 （P〈 0.01）. And the percentages of 8N cells were increased at 4, 8 and 16 h and decreased significantly at 48 h afterexposure of EL-4 cell line to 5-FU with dose of 0. 100 mg· L^-1 （P〈0.05, P〈0.01）. Conclusion The cell cycle uncoupling of EL-4 cell line could be induced by 5-FU, but Jurkat cell line could not be influenced.

关 键 词：5-氟尿嘧啶 细胞周期 解偶联 JURKAT细胞 EL-4细胞 

分 类 号：Q253[生物学—细胞生物学] R979.1[医药卫生—药品]