PLLA-PEG750纤维毡缓释紫杉醇对卵巢癌细胞SKOV3的增殖抑制作用  

Inhibitory effects of paclitaxel slowly released by PLLA-PEG750 ultrafine fibers on SKOV3 cell line

作　　者：李春生[1] 刘铜军[1] 景遐斌[2] 陈学思[2] 徐效义[2] 于惠秋[3] 

机构地区：[1]吉林大学第一医院结直肠肛门外科,吉林长春130021 [2]中国科学院长春应用化学研究所,吉林长春130022 [3]吉林大学中日联谊医院康复科,吉林长春130033

出　　处：《吉林大学学报（医学版）》2008年第4期560-564,共5页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(50373043)

摘　　要：目的:探讨担载紫杉醇纤维毡对卵巢癌细胞SKOV3的缓释效应及作用的有效性。方法:以聚左旋乳酸-聚乙二醇750(PLLA-PEG750)作为载体材料,采用电纺丝技术制备成载药率为2.5%紫杉醇纤维毡,以高效液相色谱法检测紫杉醇纤维毡药物释放情况。将其分为实验组(2.5%紫杉醇纤维毡组)、纯药组(紫杉醇作用于SKOV3组)及对照组(不加药同期培养的SKOV3组)3个组,采用MTT法筛选并计算紫杉醇纤维毡对卵巢癌SKOV3的抑瘤率,采用流式细胞术对实验组进行细胞凋亡及细胞周期分析。结果:药物释放基本符合零级动力学;担载紫杉醇纤维毡体外抑瘤率达94.59%±1.16%,接近或达到纯药组;流式细胞术表明,实验组作用于SKOV3 48 h后,G2/M期细胞百分比明显增加,48h实验组凋亡率为20.02%±0.96%,而对照组凋亡率为8.09%±0.54%,两者比较差异具有显著性(P<0.01)。结论:担载紫杉醇纤维毡具有明显缓释效应,体外作用于卵巢癌细胞SKOV3能够有效抑制肿瘤细胞增殖,阻止细胞周期进程。Objective To investigate the delayed release effect and the availability of the paclitaxel-loaded PLLA- PEG750 ultrafine fibers on ovarian cancer SKOV3 cell line. Methods Taking PLLA-PEG750 as the carrier, paclitaxel was well incorporated and dispersed uniformly in biodegradable PEG-PLLA750 fibers by using electrospinning method. The release of the drug was determined by high performance liquid chromatography. They were divided into three groups： experimental group （2.5% paclitaxel-loaded ultrafine fibers group）, pure drug group （paclitaxel on SKOV3 group）, control group （SKOV3 in the same culture period group）. Using MTT assay, the inhibitory rate of experimental group to the SKOV3 was sieved out and calculated. The apoptosis of SKOV3 cells was analyzed by flow cytometry. Results The release of the drug corresponded zero order kinetics. The inhibitory rate of the paclitaxel-loaded ultrafine fibers to SKOV3 in vitro was 94.59% ±1.16%, and approached or achieved the level in pure drug group. According to the result of flow cytometry, the percentage of G2/M of SKOV3 in experimental group 48 h after treatment was significantly up-regulated, the apoptotic rate inexperimental group （20.02 ± 0.96 %） was significantly higher than that in control group （8.09 ±0.54%） （P〈 0.01）. Conclusion The paclitaxel-loaded ultrafine fibers have conspicuous delayed release effect. It can inhibit the growth of SKOV3 cells and block the process of G2/M period in vitro.

关 键 词：紫杉醇 聚左旋乳酸-聚乙二醇750 卵巢肿瘤 缓释效应 

分 类 号：R979.1[医药卫生—药品]