羧甲基壳聚糖银对牙龈卟啉单胞菌的抑菌作用  

Antimicrobial effect of silver carboxymethyl chitosan against porphyromonas gingivalis

作　　者：佟巨慧[1] 马琳[2] 王少鹏[3] 迟立超[4] 付军权[4] 费瑞[4] 

机构地区：[1]吉林大学口腔医院牙周病科,吉林长春130041 [2]吉林大学基础医学院第三综合实验室,吉林长春130021 [3]东北师范大学生命科学院生化教研室,吉林长春130024 [4]吉林大学基础医学院细胞生物学教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2008年第4期636-639,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科技发展计划重点项目资助课题(20050413)

摘　　要：目的:研究羧甲基壳聚糖银(CMCT-Ag+)对牙龈卟啉单胞菌(Pg)的抑制作用,为研制高效无毒的抗牙周病药物提供实验依据。方法:将壳聚糖经碱化、氯乙酸修饰和离子交换制成CMCT-Ag+。采用琼脂扩散和固体平板二倍稀释法观察抑菌环直径和菌斑数,测定CMCT-Ag+对Pg的抑制作用。结果:获得了粉红色的CMCT-Ag+粉末,收率为89.4%,取代度为0.98,银离子含量为10.21%。在抑菌环实验中,当CMCT-Ag+的浓度大于0.3125g·L-1时,其抑菌环直径随浓度的增加而增大,其中40g·L-1CMCT-Ag+抑菌环直径可达9.5mm,与0.005g·L-1替硝唑相当;当CMCT-Ag+浓度大于40g·L-1时,抑菌环直径明显下降,表明CMCT-Ag+的抑菌范围为大于0.3125g·L-1。在菌斑实验中,当CMCT-Ag+浓度大于0.3125g·L-1时,培养基表面Pg的生长明显受到抑制;而当浓度大于1.25g·L-1时,培养基表面无Pg生长,表明CMCT-Ag+最低抑菌浓度为1.25g·L-1。结论:CMCT-Ag+对Pg的生长有明显抑制作用,可用于进一步研制治疗牙周炎药物。Objective To investigate the antimicrobial effect of silver carboxymethyl chitosan （CMCT-Ag^＋） on porphyromonas gingivalis （Pg）, a critical bacteria in periodontitis and provide experimental basis for developing higher efficiency and lower side-effect drug to cure periodontitis. Methods CMCT was alkalified, modified with chloracetic acid and exchanged ion to obtain CMCT-Ag^＋ complexation. The agar diffusion and plate serial dilution test was used to detect the effect of CMCT-Ag^＋ against Pg by observing cingula diameter and bacteria plaque. Results The pink CMCT-Ag^＋ was obtained, yield ratio was 89.4 %, the degree of substitution of CMCT was 0.98 and the content of Ag^＋ was 10.21% in the obtained CMCT-Ag^＋. In the agar diffusion test, when concentration of CMCT-Ag^＋ exceed 0. 3125 g · L^-1, the bacteriostasis ring diameter was increased, and at concentration of 40 g ~ L-1 , the bacteriostasis ring diameter inhibited by CMCT-Ag^＋ was 9.5 mm, that was approximation to the result induced by 0. 005 g . L-1 tinidazole. But when concentration of CMCT-Ag~ exceed 40 g ~ L-1, the bacteriostasls ring diameter was decreased. These results showed that the inhibitory concentration of CMCT-Ag^＋was more than 0. 3125 g ~ L-1. In the plate serial dilution test, when concentration of CMCT-Ag^＋ exceed 0. 3125 g ~ L-1 , the growth of Pg was obviously inhibited on medium, and when concentration of CMCT-Ag^＋ exceed 1.25 g ~ L-1 , the growth of Pg couldn＇t be found, These results indicated that the minimal inhibitory concentration （MIC） of CMCT-Ag^＋ was 1.25 g · L^-1. Oonclusion CMCT-Ag^＋ could inhibit the growth of Pg, hence it can be used for periodontal treatment.

关 键 词：羧甲基壳聚糖银 牙龈卟啉单胞菌 牙周炎 

分 类 号：R988.2[医药卫生—药品]