红花注射液对肝热缺血再灌注损伤保护作用的研究  

Protective effect of safflower solution on warm ischemia reperfusion injury of liver

作　　者：齐欣[1] 车向前[1] 王超[1] 贺志军[2] 

机构地区：[1]中南大学湘雅医学院临床医学系,湖南长沙410013 [2]中南大学湘雅二医院器官移植中心,湖南长沙410011

出　　处：《中国普通外科杂志》2008年第7期658-662,共5页China Journal of General Surgery

基　　金：中南大学大学生创新性试验计划重点项目(YA07060)

摘　　要：目的探讨红花注射液对大鼠肝热缺血再灌注损伤(IRI)的保护作用及其机制。方法选用雄性SD大鼠,随机分成4组,每组6只。S组为假手术组。缺血再灌注组(I/R组)及红花预处理组(SPC组)在缺血前30min分别经肠系膜静脉注射生理盐水或红花注射液2mL/kg,而缺血预处理组(IPC组)缺血前30min阻断血流5min。再灌注后24h检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)水平;肝组织行HE染色,观察病理组织学改变及评分(根据Suzuki标准);TUNEL法检测肝细胞凋亡;RT-PCR检测肝组织肿瘤坏死因子α(TNF-α)、巨噬细胞炎性蛋白-2(MIP-2)及细胞黏附因子-1(ICAM-1)mRNA的水平;Werstern blotting测定核转录因子κB(NF-κB)蛋白的表达。结果再灌注24 h后,与I/R组比较,SPC和IPC组血清ALT,AST水平、肝组织病理学半定量评分、肝细胞调亡指数、肝组织中TNF-α,MIP-2,ICAM-1 mRNA水平及NF-κB蛋白的表达均降低,差异均有显著性(P<0.05);SPC组与IPC组比较,上述各项指标差异均无统计学意义(P>0.05)。结论红花注射液通过下调前炎性因子TNF-α,MIP-2及ICAM-1 mRNA的水平及抗细胞凋亡作用,可减轻移植肝IRI。Objective To study the protective effect and mechanism of safflower solution on warm ischemia reperfusion injury （IRI） of liver in rats. Methods Male Sprague-Dawley rats were randomly divided as 4 groups ： Group S （ sham group ） ; group I/R （ IRI group ） ; group IPC （ ischemic preconditioning group ） ; group SPC （safflower solution preconditioning group ）. The rats were sacrificed at 24 h after reperfusion, serum levels of ALT and AST were measured and HE staining of liver tissues were made to detect rat liver histological changes and grade liver IRI （Suzuki score ）; apoptosis were monitored by TUNEL; TNF-α, MIP-2 and ICAM-1 mRNA of liver were measured by RT-PCR; NF-κB gene products of liver were detected by Western blotting. The mean value of all the assay tests was compared. Results After 24 h reperfusion,compared with group I/R, the liver function （ALT and AST） in group SPC and group IPC was significantly improved （ P 〈 0.05 ） , Suzuki scores was significantly decreased （ P 〈 0.05 ） , the level of hepatic TNF-α, MIP-2 and ICAM-1 mRNA was significantly decreased （ P 〈 0.05 ） , and the expression of hepatic NF-κB protein was also decreased （ P 〈 0. 05 ）. Conclusions Donor pretreatment with safflower solution can decrease the severity of warm IRI of liver graft by downregulation of proinflammatory cytokines TNF-α, MIP-2 and ICAM-1 mRNA and by its antiapoptotic effects.

关 键 词：肝/血液供给 缺血再灌注损伤 缺血预处理 红花 

分 类 号：R657.3[医药卫生—外科学] R277.733.3[医药卫生—临床医学]