Rho激酶对严重烧伤大鼠血清诱导的肠黏膜上皮细胞调控机制的研究  

Mechanism of Rho kinase regulating serious burn serum-induced intestinal epithelial cells in rats

作　　者：刘依凌[1] 陈传莉[1] 王裴[1] 王凤君[1] 

机构地区：[1]第三军医大学西南医院全军烧伤研究所创伤烧伤与复合伤国家重点实验室,重庆400038

出　　处：《医学研究生学报》2008年第7期693-696,共4页Journal of Medical Postgraduates

基　　金：国家自然科学基金资助项目(批准号:30571923)

摘　　要：目的：观察烧伤血清刺激下肠黏膜上皮细胞通透性的变化及Rho激酶信号转导通路所起的作用。方法：常规培养肠黏膜上皮CaCO-2细胞,分为正常对照组、烧伤血清组和烧伤血清6 h＋Y27632组,以FITC-albumin荧光强度检测法检测人肠黏膜上皮细胞在未烧伤、1、2、6、12、24 h时相点通透性变化指标;采用Western blot法检测人肠黏膜上皮细胞中肌球蛋白轻链（MLC）、磷酸化MLC（p-MLC）和Rho激酶蛋白水平在6个时相点的变化及烧伤血清6 h＋Y27632组总MLC、p-MLC及Rho激酶蛋白水平表达。结果：严重烧伤后人肠黏膜上皮细胞通透性明显增加,并呈时相依赖性变化,6-12 h达到最高;p-MLC以及Rho激酶蛋白表达均明显增加。上述蛋白表达的增强可在加入Rho激酶特异性抑制剂Y27632后取消。结论：烧伤大鼠血清能诱导肠黏膜上皮细胞内Rho激酶激活、p-MLC表达增加及肠黏膜通透性增加,抑制Rho激酶活性能逆转这一作用。Objective： To observe the changes in the permeability of intestinal epithelial cells and the role of the Rho kinase signal transduction pathway under the burn serum stimulation. Methods： Conventionally cultured CaCo-2 cells were divided into a normal control, a bum and a 6 h ＋ Y27632 group. The intestinal permeability and the mucosal protein expressions were detected 1, 2, 6, 12 and 24 hours after burn serum induction. Results： The permeability of intestinal epithelial cells was markedly enhanced in the induced serum in a time-dependent manner and reached the peak in 6 - 12 hours. The expressions of phosphorylated MLC （p-MLC） and Rho were obviously elevated, but the increasing effect was counteracted by the Rho kinase inhibitor Y27632. Conclusion： Burn rat serum can activate Rho kinase in the intestinal epithelial cells, elevate the expression of p-MLC and increase intestinal permeability, but the effect can be reversed by inhibiting the activity of Rho kinase.

关 键 词：烧伤大鼠血清 肠黏膜上皮细胞 RHO激酶 MLC磷酸化 

分 类 号：R644[医药卫生—外科学]