小滨麦易位系山农0096抗条锈基因的微卫星标记和染色体定位  

Microsatellite Marker and Chromosomal Location of the Yellow Rust Resistance Gene in Tritileymus Translocation Line Shannong0096

作　　者：王金平[1] 王洪刚[1] 赵瑾[1] 刘海燕[1] 西廷业[1] 高居荣[1] 

机构地区：[1]山东农业大学农学院国家小麦改良中心泰安分中心国家作物生物学重点实验室,泰安271018

出　　处：《分子植物育种》2008年第3期475-479,共5页Molecular Plant Breeding

基　　金：国家十一五支撑计划(2006BAD13B02)

摘　　要：从普通小麦品种烟农15与八倍体小滨麦杂交后代中选育的小滨麦种质系山农0096,综合性状优良,高抗条锈病,经鉴定为导入了滨麦草染色体的小片段易位系。通过抗性接种鉴定和遗传分析,推测山农0096的条锈病抗性由显性单基因控制,抗条锈病基因来源于滨麦草,暂将其表示为YrSn0096。利用F2分离群体分组法(BSA)对山农0096中的抗条锈病基因进行了微卫星标记分析,从1261对SSR引物中筛选出引物BARC236-4A能在滨麦草、八倍体小滨麦和山农0096中扩增出特异性DNA片段Xbarc236-4A255bp;利用该标记检测山农0096×辉县红F2群体的单株DNA,根据扩增带型,利用软件MAPMAKER3.0确定标记Xbarc236-4A与滨麦草抗条锈基因的遗传距离为5.0cM,并将该抗条锈病基因定位在4A染色体上。Shannong0096, a derivative of wheat- Octoploid Tritileymus, with useful agronomic characteristics, excellent yellow rust resistance, has been identified to be a translocation line with a small alien fragment ofL. mollis. The results of inoculation identification and genetics analysis showed that the yellow rust resistance of Shannong0096 was controlled by a single dominant gene derived from L. mollis which was temporarily designated YrSn0096. Using method of bulk segregant analysis, the Yr in Shannong0096 was identified, SSR tagged, and located. 1 261 microsatellite markers was screened, BARC236-4A was selected which has specific amplified products Xbarc236-4AE255bp in L. moUis, Octoploid Tritileymus and Shannong0096. The polymorphic marker was further used to analyze susceptible and resistant individuals of Shannong0096 ×Huixianhong F2 segregating population, and then software MAPMAKER3.0 established that the genetic distance between Xbarc236-4A and the target gene is 5.0 cM. The rust resistance gene was presumably placed on chromosome 4A.

关 键 词：小滨麦 滨麦草 易位系 抗条锈病基因 微卫星标记 

分 类 号：S512.1[农业科学—作物学]