利用分子标记检测霍山石斛不同继代次数试管苗的遗传稳定性  

The Study on the Genetic Stability of D. huoshanense Repeatedly Subcultured in vitro by Using Molecular Markers

作　　者：邱婧[1] 樊洪泓[1] 秦自清[1] 林毅[1] 蔡永萍[1] 

机构地区：[1]安徽农业大学生命科学学院,合肥230036

出　　处：《分子植物育种》2008年第3期532-536,共5页Molecular Plant Breeding

基　　金：安徽省教育厅自然科学重点科研项目(2006KJ054A)

摘　　要：分别以霍山石斛成熟种子、茎段和愈伤组织为试管苗的初始材料进行继代培养,利用RAPD、ISSR以及DALP3种分子标记方法对不同继代次数(1￣4代)的霍山石斛试管苗进行分析,结果表明:以成熟种子和茎段为材料的继代培养,其试管苗后代在所检测的范围内未探查到变异,具有较高的遗传稳定性。以愈伤组织为材料的继代培养,其试管苗后代从第4代开始,部分引物带型发生变化,但变化甚微,仅是带型强弱的改变。以愈伤组织为材料的继代培养物种内存在一定的变异,但在4代以内仍可稳定遗传。3种继代方式比较之下,以成熟种子和茎段为材料的继代培养是更能稳定遗传的组培快繁方式。The genetic stability ofDendrobium huoshanense repeatedly subcultured in vitro for one to four times with three different original materials of mature seed, stem segment, and callus, was examined by using RAPD, ISSR and DALP. The results showed that no variation was detected within the descends ofD. huoshanense repeatedly subcultured in vitro with materials of mature seed and stem segment, which revealed that high genetic stability was maintained. The slight variations of some primer bands were detected since the 4th descends, which was only the change of the clearance of the band, using the material of callus. The genetic stability ofD. huoshanense repeatedly subcultured in vitro with the material of callus was maintained within four descends, though some variations were found. With the comparison of the three subculturing methods, it indicated that repeatedly subculturing with the materials of mature seed and stem segment were better rapid propagation ways to maintain the genetic stability of D. h uo s hane nse .

关 键 词：霍山石斛 试管苗 继代次数 遗传变异 分子标记 

分 类 号：S567.239[农业科学—中草药栽培]