绿竹咖啡酰辅酶A-O-甲基转移酶基因的克隆与分析  

Cloning and Characterization of CCoAOMT Gene from Bambusa oldhamii

作　　者：李雪平[1] 高志民[1] 彭镇华[2] 岳永德[1] 

机构地区：[1]国际竹藤网络中心国家林业局竹藤科学与技术重点开放实验室,北京100102 [2]中国林业科学研究院林业研究所,北京100091

出　　处：《分子植物育种》2008年第3期587-592,共6页Molecular Plant Breeding

基　　金：国家林业局948项目(2005-4-38);国际竹藤网络中心基本科研业务费专项(618-13);国家林业局重点林业科学技术研究项目(2007-01)

摘　　要：咖啡酰辅酶A-O-甲基转移酶(CCoAOMT)是木质素生物合成过程中的一个关键酶类。采用RT-PCR及RACE方法从绿竹中分离了CCoAOMT家族的一个基因,命名为BoCCoAOMT1(GenBank注册号为EF028662)。BoCCoAOMT1的cDNA全长873bp,含有一个780bp的读码框,编码一个259aa的蛋白,分子量约为29kD。序列分析结果表明,BoCCoAOMT1的编码肽链含有CCoAOMT类基因所有的保守序列元件,与禾本科植物水稻的CCoAOMT类基因有着很高的一致性,达到90.0%。系统进化树分析表明,BoC-CoAOMT1与水稻的CCoAOMT亲缘关系较近。Caffeoyl-CoA O-methyltransferase （CCoAOMT） is a key enzyme in the biosynthesis of lignin. A gene of CCoA OMT family was cloned by RT-PCR and RACE from Bambusa oldhamii and named as BoCCoA OMT1 （GenBank accession number： EF028662）. BoCCoA OMT1, with the cDNA length of 873 bp, contained an open reading frame of 780 bp which encoded a polypeptide of 259 amino acids with predicted molecular mass of 29 kD. The results of sequence analysis showed that the deduced polypeptide contained all the conserved sequences of CCoA OMT family and it was highly homologous to CCoAOMT proteins from rice with 90.0% identity. Phylogenetic tree analysis also indiated that BoCCoAOMT1 was more related to the CCoAOMT in rice.

关 键 词：绿竹 咖啡酰辅酶A-O-甲基转移酶 克隆 系统进化树分析 

分 类 号：Q943.2[生物学—植物学]