新一代测序技术及其对水稻分子设计育种的影响  

The Next Generation Sequencing and its Effect on the Rice Molecular Design Breeding

作　　者：周德贵[1] 赵琼一[2] 付崇允[1] 李宏[1] 蔡学飞[2] 罗达[2] 周少川[1] 

机构地区：[1]广东省农业科学院水稻研究所,广州510640 [2]中国科学院上海植物生理生态研究所,上海200032

出　　处：《分子植物育种》2008年第4期619-630,共12页Molecular Plant Breeding

基　　金：国家-广东联合自然科学基金项目(U0631002;U0731007);国家863计划资助项目(2006AA1021F7);国家科技成果转化项目(2007GB2E000242);国家跨越计划资助项目(2008-29);农业部948项目(2006-G-1);广东省农科院科技专项(08-专项-01)资助

摘　　要：本文概述了以边合成(或连接)边测序为特征的新一代测序技术的基本原理以及现有的Roche(454)GSFLXsequencer、Illumina genome analyzer(Solexa)、Applied Biosystems SOLiDsequencer、HeliScope Sequencer等4种新一代测序平台的发展历史、测序流程、优缺点和测序成本等,并展望了新技术的发展可能对以水稻为代表的农作物大规模分子设计育种产生的影响。本文还提出通过对水稻核心种质育种材料体系的全基因组测序,开发功能性分子标记和特异基因芯片,缩短将基础研究成果应用于实际育种生产的时间,为真正进入分子设计育种时代奠定基础。This review is about the principle of the next generation sequencing and the history, workflow, advantages and cost of the four sequencing platforms, Roche （454） GS FLX sequencer, Illumina genome analyzer （solexa）, Applied Biosystems SOLiD sequencer, HeliScope Sequencer, developed on the principle of Sequencing by Synthesis or Ligation. It was discussed the effect of new technology on rice molecular design breeding. It is suggested that the whole genome sequencing of a series of rice core germplasm, development of functional molecular markers and specific microarrys will accelerate the application of the basic research results to the breeding and lays the foundation for molecular design breeding.

关 键 词：水稻 新一代测序 分子设计育种 

分 类 号：S511[农业科学—作物学]