NaHCO3胁迫下星星草根特异表达蛋白初步鉴定  

Preliminary Identification of Root Specific-Expressed Protein in Puccinellia tenuiflora under NaHCO_3 Stress

作　　者：杨春雪[1] 罗秋香[1] 卓丽环[1] 柳参奎[1] 

机构地区：[1]东北林业大学盐碱地生物资源环境研究中心,哈尔滨150040

出　　处：《分子植物育种》2008年第4期669-674,共6页Molecular Plant Breeding

基　　金：国家863计划(2002AA241111);黑龙江省杰出青年基金(JC200609);东北林业大学青年科研基金项目(07047)

摘　　要：盐胁迫是影响全世界作物产量的主要非生物胁迫之一,东北松嫩平原的苏打盐碱土含有相对较高的碳酸盐(NaHCO3和Na2CO3)。与NaCl胁迫相比,碳酸盐胁迫对植物的影响具有更加复杂的特性,除Na+对植物的生长发育产生影响外,高pH也强烈抑制植物多种代谢过程。星星草是一种广泛分布于我国北方的天然耐盐碱植物,在含高Na+和高pH值的土壤中能够生长良好,本文中,我们以星星草为实验材料,对NaHCO3与NaCl胁迫下星星草根蛋白质的双向电泳图谱进行了初步的比较与分析,以水(CK1)、NaCl(CK2)为对照,初步探查了NaHCO3胁迫特异诱导表达蛋白。实验结果表明,大部分蛋白质点在凝胶上的相对位置和丰度变化不明显,有7个蛋白质点与NaCl胁迫相比为NaHCO3胁迫特异诱导表达蛋白,5个点在两种盐胁迫下丰度均上调,但在NaHCO3胁迫下上调更明显。这些蛋白参与基因表达调控及能量代谢等过程。Soil salinity is one of the major abiotic stresses that limits the development of agriculture worldwide. The carbonates （NaHCO3, Na2CO3） content in Songnen plain of Northeast China is relative high. Compared with NaCl stress, the effect of NaHCO3 stress on plant is more complex. Besides the effects of Na^＋ from carbonates on plant growth and development, their pH also strongly restricts many plant metabolic processes. Puccinellia tenuiflora is a wild salinity-alkali tolerant plant widely distributed in the northeast of China, and it can grow well in the soil with high Na^＋ concentration and high pH. Two-dimensional gel electrophoresis maps of Puccinellia tenuiflora root protein under NaCl and NaHCO3 stress were primarily compared and analyzed in this paper. Expression of specific protein induced by NaHCO3 was explored, taken water （CK1） and NaCl （CK2） stress as comparison. Experiment results showed that most of the protein spots did not change a lot on the gel in their relative position or abundance. Compared with NaCl stress, 7 protein spots were specific protein induced by NaHCO3. 5 protein spots were up-regulated in their abundance under both two kinds of salt stress, with those under NaHCO3 stress raised more evident. These proteins participate in the regulation of gene expression and energy metabolic processes.

关 键 词：星星草 NAHCO3胁迫 特异表达 蛋白质 

分 类 号：S184[农业科学—农业基础科学]