水稻成熟胚培养高效再生系统的创新  

Innovation of High Effective Regeneration System for Matured Embryo in vitro Culture of Rice

作　　者：黄赛麟 李东宣[1] 甘树仙[1] 朱建荣[1] 李娟[1] 梁晶[1] 陈丽娟[1] 

机构地区：[1]云南农业大学农学与生物技术学院,昆明650201

出　　处：《分子植物育种》2008年第4期801-806,共6页Molecular Plant Breeding

基　　金：云南省应用基础研究重点项目(K2100295)

摘　　要：本文测试了不同消毒方式、愈伤组织诱导、绿苗分化及生根条件对不同类型水稻成熟胚培养再生成株的影响。研究结果表明:改良培养基比基本培养基处理效果更好,在改良培养基N6I愈伤诱导、MSR绿苗分化和MSC生根条件下,籼稻(O.sativa indica cv.滇屯502)、粳稻(O.sativa japonica cv.日本晴)、籼粳杂种F1(滇屯502/日本晴)和非洲栽培稻(O.glaberrima cv.RG105)的诱导率分别为96%、100%、98%和100%,成苗率分别为264%、286%、216%和224%。以YuhanClorox(4%NaClO)稀释一半消毒1h效果最好。由此建立了一套非常高效、可靠、重复性好的适用于不用基因型水稻成熟胚再生系统,为水稻遗传转化和多倍体化的顺利进行奠定了基础。possible factors may affect the regeneration of rice plantlets from matured embryo in vitro culture, such as methods of sterilizing and callus induction, differentiation and regeneration of green plantlets, and conditions for plantlet rooting, of different genotypes of indica and japonica rice, and rice cultivar from Africa were investigated.The main results showed that the treatment effect of modified medium was better than that of the basic ones, and the sterilizing of matured embryos with half of Yuhan Clorox （4% sodium hypochlorite） for one hour showed the best action. By use of modified medium, the frequency of callus induction was 96%, 100%, 98%, and 100%, frequency of rooting was 264%, 286%, 216%, and 224%, in indica （Diantun502）, japonica （Nipponbare）, F1 of indica/japonica, and African （RG105） cultivars, respectively.Consequently, a high efficient regeneration system for in vitro culture of matured embryos of rice was established, which would be reliable and repeatable for different genotypes of rice cultivars and could further be facilitated for smooth genetic transformation and polyploidization of rice.

关 键 词：水稻 成熟胚 组织培养 高效再生系统 

分 类 号：S511[农业科学—作物学]