我国南方某省猪繁殖与呼吸综合症病毒分离株分子流行病学分析  

Molecular Epidemiological Analysis of Porcine Reproductive and Respiratory Syndrome Virus Isolates in South China

作　　者：吴发兴[1] 解生亮[1,2] 张燕霞[1] 张志[1] 李晓成[1] 刘道新 黄保续[1] 

机构地区：[1]中国动物卫生与流行病学中心,山东青岛266032 [2]东北农业大学动物医学学院,黑龙江哈尔滨150030 [3]湖南省兽医总站,湖南长沙410007

出　　处：《中国动物检疫》2008年第8期23-25,共3页China Animal Health Inspection

基　　金：科技部十一五科技支撑计划"高致病性猪蓝耳病紧急防控技术研究"提供资助

摘　　要：根据GenBank上发表的猪繁殖与呼吸综合征病毒(PRRSV)VR-2332的基因序列设计并合成了一对针对ORF5基因的引物,通过RT-PCR方法对我国南方某省部分地区分离的14个PRRSV分离株进行扩增,获得了大约773bp的DNA片断,将目的片断与pMD18-T载体进行连接并测序。应用DNAStar分析所测序列,并与LV、VR-2332、CH-1a、BJ-4、HB-1、HB-2、JX-1等毒株的序列进行比较。核苷酸序列分析表明:14株病毒的与JX-1ORF5基因同源性高达99.0%～99.8%,与CH-1a、HB-1、HB-2同源性达到91.4%～94.9%,与VR-2332、BJ-4同源性达到86.1%～87.6%,与LV同源性仅为55.2%～55.7%。氨基酸序列分析表明:与JX-1同源性高达97.5%～99%;与VR-2332、CH-1a、BJ-4、HB-1、HB-2同源性达到85.6%～94.5%;与LV同源性仅为55.7%～56.2%。可知这14株病毒与JX-1遗传关系较近,可归属同一基因亚群。A pair of primers specific to the ORF5 gene were designed and synthesized according to the published genome sequence of PRRSV VR-2332 on Genbank and an RT-PCR was developed to amplify 14 PRRSV isolates from parts of Hunan province, obtaining DNA fragments of approximetely 773bp. The target fragment was connected to pMD18-T vector and sequenced. The sequence was analysed by menas of DNA star software and compared with those of LV, VR-2332, CH-la, JB-4, HB-1, HB-2, JX-1 strains. Nueleotide sequence analysis showed that the ORF-5 gene holomology of the 14 isolates was upto 99.0%-99.8% with JX-1;91.4%-94.9% with CH-la, HB1 and HB2; 86.1%-87.6% with VR-2332, BJ-4, and only 55.2%-55.7% with LV and the amino acid sequence analysis showed that the their homology was 97.5%-99% with JX-1; 85.6%-94.5% with VR-2332, CH-1a, BJ-4, HB1 and HB2; and only 55.7%-56.2% with LV. It was suggested that the PRRSV 14 isolates are closely related to JX-1 genotieally and can be grouped in the same subtype.

关 键 词：猪繁殖与呼吸综合征病毒 ORF5基因 进化树 分子流行病学 

分 类 号：S858.3[农业科学—临床兽医学] S858.28[农业科学—兽医学]