中药有效成分Nordosin增强FK506体外抑制淋巴细胞IL-2分泌作用的研究  

Nordosin enhanced inhibition of FK506 on the secretion of IL-2 by lymphocyte derived from spleen of mice in vitro

作　　者：杜隽铭[1] 吴萍[1] 孙丽娟[2] 刘建文[2] 李济宇[1] 全志伟[1] 

机构地区：[1]上海交通大学医学院附属新华医院普外科,上海200092 [2]华东理工大学药学院,上海200237

出　　处：《中国临床药理学与治疗学》2008年第6期610-613,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金项目(30600598);上海市青年科技启明星计划项目(06QA14034)

摘　　要：目的:观察中药有效成分Nordosin对FK506体外抑制致炎细胞因子作用的影响,探讨Nordosin在移植免疫领域存在的潜在应用价值。方法:建立ConA激活小鼠脾淋巴细胞增殖模型,采用ELISA和RT-PCR方法,测定Nordosin与FK506联合应用对淋巴细胞的IL-2蛋白和mRNA表达的影响;同时,运用流式细胞检测T淋巴细胞CD25+表达的变化。结果:Nordosin与FK506联合用药可以抑制小鼠脾淋巴细胞的IL-2分泌(P<0.01),IL-2 mRNA表达以及T淋巴细胞CD25+表达也显著降低(P<0.01),其作用强于单独使用FK506组(P<0.01)。结论:Nordosin可以增强FK506抑制体外淋巴细胞IL-2分泌及其受体表达的作用,Nordosin可望成为一种免疫抑制辅助用药。AIM： To investigate the effect of Nordosin in inhibiting proinflammatory cytokine with FK506 in vitro and potential applied value in the transplantation immunity.METHODS： ConA-stimulated T-cell proliferation model was made.Then,the expression of IL-2 both in protein and mRNA lever were investigated by ELISA and RT-PCR.Furthermore,the expression of IL-2 receptor,CD25^＋ on surface of T lymphocyte was also measured by FCM.RESULTS： Compare with those by FK506 use along,Nordosin enhanced the inhibition of FK506 on the expression of IL-2 significantly（P〈0.01）.The expression of IL-2 mRNA and its receptor CD25^＋ were also inhibited by their combining medication（P〈0.01）.CONCLUSION： Nordosin could enhance the inhibition of FK506 on IL-2 secretion significantly,Nordosin maybe become an immunodepression assistant medication to enlarge the pharmacological effect of FK506.

关 键 词：Nordosin FK506 CD25^+ IL-2 移植免疫 

分 类 号：R965.2[医药卫生—药理学]