糖腈乙酰酯衍生气相色谱法测定土壤水解性单糖  

Analysis of Soil Hydrolyzed Monosaccharides by Gas Chromatography as Aaldononitrile Acetates Derivatives

作　　者：张威[1] 何红波[1] 张明[1] 张旭东[1] 

机构地区：[1]中国科学院沈阳应用生态研究所陆地生态过程重点实验室

出　　处：《土壤通报》2008年第4期913-916,共4页Chinese Journal of Soil Science

基　　金：国家自然基金重点项目(40535028);中国科学院知识创新工程重要方向资助项目(KZCX3-SW-433);沈阳应用生态研究所创新领域前沿项目资助(SLYQY0602)

摘　　要：通过对糖腈乙酰酯衍生条件的优化,建立了一种测定土壤水解性单糖的糖腈乙酰酯衍生气相色谱分析方法。8种单糖线性范围为10～640!g,检出限(3S/N)在1.4～4.2ng之间,样品加标平均回收率为100.1%,相对标准偏差(RSD)为1.5～6.3%。试验结果表明该方法操作简便快速、灵敏度高,可准确可靠地应用于土壤单糖含量的测定。This paper established a method for the determination of soil monosaccharides by gas chromatography as aldononitrile acetates derivatives after optimizing the derivativation condition including the amount of derivatization reagent, reaction time, and the volume of extraction reagents. Linearity of the 8 monosaccharides was in the range of 10 -6401μg and detection limit of the method was in the range of 1.4-4.2ng. The averaged recovery of the 8 monosaccharides was 100.1% and the value of RSD was in the range of 1.5%-6.3%. These results showed that this method was simple, sensitive, and accurate for the analysis of soil samples.

关 键 词：单糖 糖腈乙酰酯衍生 土壤 气相色谱 

分 类 号：S151.93[农业科学—土壤学]