太空搭载‘热研2号’柱花草后代RAPD多态性分析  

Polymorphic Analysis of RAPD in Stylosanthes guianensis 'Reyan No.2' after Spaceship-carried Seed Induction

作　　者：白昌军[1] 刘国道[1] 姚庆群[1] 虞道耿[1] 陈志权[1] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所,海南571737

出　　处：《草地学报》2008年第4期336-340,共5页Acta Agrestia Sinica

基　　金：国家科技基础条件平台建设项目(2005DKA21000)

摘　　要：以返回式卫星搭载的‘热研2号’柱花草（Stylosanthes guianensis（Aubl．）Sw．）种子为材料，经过5年地面种植和选择，获得26个变异株系。利用RAPD分子标记技术对26个变异株系和2个对照‘热研2号’与‘热研5号’柱花草进行遗传多样性研究。从50个随机引物中筛选出11个，共扩增出77条带，其中53条为多态性带，占68．8％，平均每个引物扩增出多态性带4．8条；采用Nei72方法计算出不同材料间的遗传距离，其变化范围为0．056～0．509；利用NTSYS软件进行非加权组法（UPGMA）聚类分析，建立‘热研2号’柱花草太空育种后代材料间的分子系统树状图；并以相似系数0．77为标准，将28份材料分为5类。Twenty-six mutants were obtained from the 5-year plantation of the seeds of Stylosanthes guianensis （Aubl.） Sw. cv. ‘Reyan No. 2’ which were carried into outer space by a recoverable satellite in 1996. The genetic diversity of the 26 mutant lines and the contral varieties （S. guianensis cv. Reyan No. 2 and S. guianensis cv. Reyan No. 5） was analyzed by RAPD using 11 primers from 50 random primers. There were 53 polymorphic DNA bands （68.8%） among the 77 DNA bands amplified by RAPD. The av- erage number of polymorphic DNA bands per primer was 4.8, and the range of genetic distance among these lines varied from 0.056 to 0. 509 as assessed by Nei＇s 72. A DNA molecular tree diagram was estab- lished base on UPGMA analysis by NTSYS software and the 28 materials of S. guianensis were classified into five groups according to a standard of similarity coefficient at 0.77 in the dendrogram.

关 键 词：热研2号柱花草 太空育种 RAPD 遗传多样性 聚类分析 

分 类 号：S812[农业科学—草业科学]