大鼠去势后肾上腺与前列腺中Cyp17a1基因的表达  

Cyp17a1 Gene Expression in Rat′s Adrenal and Prostate After Orchiectomy

作　　者：王琬[1] 何永鑫[2] 陈君[2] 李雪[2] 罗国安[1] 王义明[2] 

机构地区：[1]华东理工大学药学院,上海200237 [2]清华大学化学系,北京100084

出　　处：《高等学校化学学报》2008年第7期1349-1351,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:30772169)资助

摘　　要：通过定量检测大鼠去势前后肾上腺和前列腺中Cyp17a1基因的表达,试图从分子水平解释去势手术后大鼠机体内雄性激素水平的变化.In this paper the change law of Cypl7al gene expression in the process was studied, in which the rats＇ adrenal secretes androgen after orehiectomy. The Cypl7al gene expression in adrenal and prostate tissues was detected via real-time fluorescent quantitative PCR on different time points, which were before orehiectomy and 1 week, 2 weeks, 3 weeks, 4 weeks after orchiectomy, respectively. The results indicate that Cypl7al gene expression in adrenal would increase after orchiectomy, and reach the maximum when 3 weeks after. That Cypl7al gene exists in rat＇s prostate tissue was discovered for the first time. The conclusion is that adrenal have the function of compensation, increasing the secretion of androgen after orchiectomy. It is presumed that prostatecan synthesizes DHEA as well.

关 键 词：Cypl7al 肾上腺 前列腺 实时荧光定量PCR 

分 类 号：O657[理学—分析化学] Q786[理学—化学]