微量热法研究头孢菌素对大肠杆菌微生物活性的影响  

A Study of the Influence of Cephalosporins on the Microbial Activity of E.coli by Microcalorimetry

作　　者：杨黎妮[1] 孙立贤[1] 徐芬[1] 赵宗保[2] 梁建国[3] 

机构地区：[1]中国科学院大连化学物理研究所材料热化学实验室 [2]中国科学院大连化学物理研究所生物质高效转化课题组,大连116023 [3]湖南省药品检验所,长沙410001

出　　处：《高等学校化学学报》2008年第7期1412-1415,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:50671098,20473091,20573112)资助

摘　　要：用微量热法测定了两种头孢菌素头孢哌酮钠(CFZ)和头孢哌酮钠舒巴坦钠(CFZ-SBT)在37℃时对大肠杆菌DH5α代谢作用的影响.根据产热曲线分别获得了大肠杆菌DH5α在不同浓度的头孢哌酮钠和头孢哌酮钠舒巴坦钠作用下的生长速率常数(k)、抑制率(I)、最大产热功率(Pm)以及最大产热功率所对应的时间tm等热动力学参数.研究结果表明,头孢哌酮钠和头孢哌酮钠舒巴坦钠对大肠杆菌的致死量分别为0.1和0.25μg/mL.通过研究k,I,Pm,tm和浓度(c)间的关系发现,舒巴坦钠的加入没有增加头孢哌酮钠对大肠杆菌DH5α的抑制作用.The effects of Cefoperazone Sodium（CFZ） and Cefoperazone Sodium ＆ Sulbactam Sodium for in- jection（CFZ-SBT） on the metabolism of Escherichia coli （.E. coli） DH5α were studied by microcalorimetry. The microbial activity was recorded as power-time curves through an ampoule method with a TAM air isothermal microcalorimeter at 37℃. The parameters such as the growth rate constant（k）, inhibitory ratio（I）, the maximum power output（Pm） and the time（tm） corresponding to the maximum power output were obtained. The lethal doses of CFZ and CFZ-SBT were calculated as 0. 1 and 0. 25 μg/mL, respectively. Moreover, according to the relationship between k, Pm, tm and c, the conclusion could be made that the CFZ-SBT cannot improve the inhibitory effect on E. coli DH5α in comparison with the CFZ.

关 键 词：微量热法 抑制作用 头孢菌素 大肠杆菌 

分 类 号：O642.3[理学—物理化学]