异丙酚对急性和慢性代谢性酸中毒大鼠红细胞内pH的影响  

Effect of propofol on intracellular pH of erythrocytes in rats with acute or chronic metabolic acidosis

作　　者：袁媛[1] 张诗海[2] 姚尚龙[2] 王贤东[1] 王冬[1] 

机构地区：[1]甘肃省人民医院麻醉科,兰州市730030 [2]华中科技大学同济医学院附属协和医院麻醉科

出　　处：《中华麻醉学杂志》2008年第6期530-533,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金资助项目（30271255）

摘　　要：目的观察异丙酚对急性和慢性代谢性酸中毒大鼠红细胞内pH（pHi）的影响。方法Wistar大鼠，雌雄不拘。第一部分采用静脉输注HCl的方法制备急性代谢性酸中毒模型，模型制备成功的40只大鼠随机分为4组（n=10）：10％脂肪乳10ml·kg^-1·h^-1组（F组）、碳酸酐酶（CA）抑制剂乙酰唑胺20mg·kg^-1·h^-1组（Z组）、异丙酚10mg·kg^-1·h^-1组（P1组）和异丙酚20mg·kg^-1·h^-1组（P2组）。第二部分采用管饲NH4Cl的方法制备慢性代谢性酸中毒模型，模型制备成功的40只大鼠随机分为4组（n=10），处理情况同第一部分。分别于给药前、给药1、3、6h时采集动脉血样，测定红细胞pHi、CA活性及钠氢交换体-1（NHE-1）活性。结果第一部分与F组比较，Z组CA活性降低（P〈0．05或0．01），P1组和B组pHi、CA活性及NHE-1活性差异无统计学意义（P〉0．05）。第二部分与F组比较，Z组、P1组和P2组pHi和CA活性降低（P〈0．05或0．01）；与Z组比较，P1组和P2组phi和CA活性降低（P〈0．05或0．01），NHE-1活性差异无统计学意义（P〉0．05）。结论静脉输注异丙酚10、20mg·kg^-1·h^-1可导致慢性代谢性酸中毒大鼠红细胞pHi降低，其机制与抑制CA活性有关；而对急性代谢性酸中毒大鼠红细胞pHi无影响。Objective To investigate the effect of propofol on erythroeyte intraeellular pH （pHi） in rats with acute or chronic metabolic acidosis. Methods Adult Wistar rats of both sexes weighing 290-330 g were used in this study. In the first part, acute metabolic acidosis was induced by 0. 15 mol/L HCl （4 mmol/kg） infusion via tail vein for4 h and then forty rats were randomly divided into 4 groups （n = 10）： eontrol group （F） 10% fat emulsion 10 ml·kg^-1·h^-1 , aeetazolamide 20 mg·kg^-1·h^-1 group （Z）, propofol 10 mg·kg^-1·h^-1 group （P1） and propofol 20 rag＂ kg- l. h - 1 group （ P2 ）. In the seeond part, ehronie metabolie aeidosis was indueed by drinking water containing NH4 Cl 0.28 mol/L （535 mg/kg） for 5 days and then forty rats were randomly divided into 4 groups （ n = 10） receiving the same drugs respeetively as that of the first part. Blood samples were taken from femoral artery immediately before and at 1, 3 and 6 h during drug administration for determination of carbonic anhydrase （CA） activity, pHi and sodium-hydrogen exchanger-1 （NHE-1） aetivity. Results In the first part, eompared with group F, CA aetivity was signifieantly deereased after drug administration in group Z （P 〈 0.05 or 0.01）, while there was no significant differenee in pHi and CA and NHE-1 activity after drug administration in group P1 and P2 （P 〉 0.05）. In the seeond part, pHi and CA aetivity were signifieantly decreased after drug administration in group Z, P1 and P2 than in group F （P 〈 0.05 or 0.01 ）; pHi and CA activity were significantly decreased after drug administration in group PI and P2 than in group Z, while there was no significant difference in NHE-1 activity after drug administration between group Z, P1 and P2 （ P 〉 0.05 ）. Conclusion pHi can be decreased by infusion of propofol at 10 and 20 mg·kg^-1·h^-1 in rats with chronic metabolic acidosis by inhibition of CA, but is not affected by propofol infusion in rats with acute metabolic acidosis.

关 键 词：二异丙酚 酸中毒 红细胞 氢离子浓度 

分 类 号：R614[医药卫生—麻醉学]