乙二胺四醋酸使耐药菌恢复对抗菌药物敏感性的研究  

Study on recovery of antibacterials sensitivity of the drug resistant bacteria by EDTA

作　　者：庞晓军[1] 莫国艳[2] 利春红[1] 韦华 

机构地区：[1]钦州市第二人民医院,广西钦州535000 [2]广西医科大学2004级博士生 [3]广西桂林市人民医院,广西桂林541000

出　　处：《中国医院药学杂志》2008年第14期1182-1184,共3页Chinese Journal of Hospital Pharmacy

摘　　要：目的:探索恢复多重耐药细菌敏感性的方法。方法:通过琼脂平皿二倍稀释法加入乙二胺四醋酸(EDTA)测定各药物对产ESBLs多重耐药的肺炎克雷伯杆菌及大肠埃希杆菌的MIC的。在此基础上,采用阿米卡星注射液80mg,qd+左旋氧氟沙星注射液400mg,qd+EDTA注射液1g,qd(其中0.5g加入阿米卡星注射液,另外0.5g加入左旋氧氟沙星注射液中,EDTA连续使用3d后停用2d为一疗程)治疗产ESBLs多重耐药的肺炎克雷伯杆菌及大肠埃希杆菌感染25例患者,进行10d治疗后观察疗效。结果:大多数受试药物加入EDTA后对产ESBLs的肺炎克雷伯杆菌和大肠埃希杆菌的MIC都有所降低并恢复敏感性,但氨曲南、头孢噻肟仍然耐药。而进行临床治疗探索也表明,阿米卡星注射液联合左旋氧氟沙星注射液并加入EDTA注射液治疗产ESBLs的肺炎克雷伯杆菌和大肠埃希杆菌的感染具有较高的有效率。结论:在各抗菌药物中加入乙二胺四醋酸为治疗多重耐药细菌的新途径。OBJECTIVE To explore methods for recovering sensitivity for the multi-drug resistant bacteria. METHODS EDTA was added to the agar dish to determine MIC of Klebsiella pneumoniae and the Escherichia coli with multi-drug resistence. Amikacin injection 80 Mg, qd ＋ levofloxacin injection 400 Mg, qd ＋ EDTA injection 1 g, qd （0. 5 g added to the Amikacin injection, another 0. 5 g added to the Levofloxacin injection,for 3 days,stops using for 2 day as a treatment course） were used for 25 patients for 10 days, the curative effect was observed. RESULTS The sensitivity was recovered for most of the tested drugs when EDTA was added except aztreonam, cefotaxime, clinical results indicated that amikacin and levofloxacin injections had better effect when EDTA was added. CONCLUSION It is a new way to treat the multi-drug resistance.

关 键 词：多重耐药 EDTA肺炎克雷伯杆菌 大肠埃希杆菌 

分 类 号：R969[医药卫生—药理学]