两种有胃真骨鱼胃肠胰系统中内分泌细胞的鉴别与定位  

LOCALIZATION AND IDENTIFICATION OF ENDOCRIN CELLS IN THE GASTROENTEROPANCREATIC SYSTEM OF TWO SPECIES OF STOMACH CONTAINING TELEOSTS

作　　者：方之平[1,2,3] 潘黔生[1,2,3] 聂秀云 白雪梅[1,2,3] 

机构地区：[1]华中农业大学畜牧兽医学院 [2]华中农业大学水产学院 [3]武汉市洪山区水产科学研究所

出　　处：《华中农业大学学报》1997年第6期594-598,共5页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金

摘　　要：使用４种哺乳动物抗血清；胰高血糖素（ｇｌｕｃａｇｏｎ，Ｇｌｕ），胃泌素（Ｇａｓｔｒｉｎ，Ｇａｓ），生长抑素（ｓｏｍａｔｏｓｔａｔｉｏｎ，Ｓｏｍ）５－羟色胺（５－ｈｙｄｒｏｘｙｔｒｙｐｔａｍｉｎｅ，５－ＨＴ），采用过氧化物酶标记的链霉亲和素（Ｓｔｒｅｐｔａｖｉｄｉｎ－Ｐｅｒｏｘｉｄａｓｅ，简称Ｓ－Ｐ法）免疫细胞化学技术，对尼罗非鲫（Ｔｉｌａｐｉａｎｉｌｏｔｉｃａ，简称非鲫）和短盖巨脂鲤（Ｃｏｌｏｓｏｍａｂｒａｃｈｙｐｏｍｕｍ，简称白鲳）的胃肠胰（Ｇａｓｔｒｏ－Ｅｎｔｅｒｏ－Ｐａｎｃｒｅａｔｉｃ，ＧＥＰ）内分泌系统进行了研究。结果表明：２种鱼的消化道和胰岛都不同程度地存在ＡＰＵＤ（ＡｍｉｎｅＰｒｅｃｕｒｓｏｒＵｐｔａｋｅａｎｄＤｅｃａｒｂｏｘｙｌａｔｉｏｎ）细胞的分布。除了Ｇｌｕ在消化道中为阴性外，其余３种抗体在２种鱼的消化道中均有阳性特异性反应，其中５－ＨＴ细胞最多，Ｓｏｍ次之，Ｇａｓ仅存在于白鲳贲门上皮细胞之间。在２种鱼的胰岛中均鉴别出Ｇｌｕ细胞，Ｓｏｍ细胞仅分布在非鲫的胰岛中。本文描述了不同部位内分泌细胞的分布密度和形态学特征并讨论了它们分布的特点。By using 4 kinds of antisera raised against mammalian hormones: glucagon (Glu), gastrin (Gas), somatostatin (Som) and 5hydroxytryptamine (5HT), the gastroenteropancreatic (GEP) endocrine system of nile tilapia (Tilapia nilotica) and freshwater pomfret (Colossoma brachypomum) were studied with an immunocytochemical technique of streptavidin labelled peroxidase. The results showed that there were distribution of these amine precursor uptake and decarboxylaiton (APUD) cells in the digestive tract mucosa and pancreatic island in varying degress. Positive specific reaction of 3 kinds of antisera was found in the digestive tract of 2 fish species except glucagon antiserum. The 5HT positive reactive cells were the largest in the disgestive tract and the somatostatincells were the second. The gastrincells located only among the cardiac epithelial cells space. The glucagoncells were identified in the pancreatic island of the two fish species. The somatostatincells were only observed in the pancreatic island of nile tilapia. The morphologic characteristics of endocrine cells were described and their distribution feature were discussed.

关 键 词：尼罗非鲫 鲤 胃肠胰系统 内分泌细胞 

分 类 号：S91[农业科学—水产科学] Q959.4[生物学—动物学]