日本血吸虫重组抗原Sj GST Paramyosin 和 Sj 23对绵羊的保护性免疫试验  

Protective immunity test in sheep by recombinant antigen Sj GST Paramyosin and Sj23 of Schistosoma japonicum

作　　者：施福恢[1] 沈纬[1] 田锷[1] 叶萍[1] 蔡学忠[1] 钱承贵[1] 林邦发[1] 傅志强[1] 刘金明[1] 石耀军[1] 陈永军[1] 李浩[1] 张正达[1] 吴祥甫[1] 蔡幼民 M.Taylor M.C.Huggins Q.D.Bickle 

机构地区：[1]中国农业科学院上海家畜寄生虫病研究所 [2]英国伦敦大学卫生与热带医学院 [3]中国科学院上海生物化学研究所

出　　处：《中国兽医科技》1997年第11期3-6,共4页Chinese Journal of Veterinary Science and Technology

基　　金：中国总理专项研究基金

摘　　要：１９９４～１９９５和１９９７年应用日本血吸虫３类候选疫苗分别免疫绵羊，各组的减虫率、粪便虫卵减少率和组织（肝、大肠、小肠）的虫卵减少率分别是：虫体副肌球蛋白（ｎ－ｐａｒａｍｙｏｓｉｎ）组１７．４％～５５．３％，１４．６％～６８．１％和４８．９％～７６．７％；重组副肌球蛋白（ｒ－ｐａｒａｍｙｏｓｉｎ）组４１．４％～４４．２％，１５．９％～２５．１％和４１．１％～４８．０％；Ｓｊ２６谷胱甘肽Ｓ转移酶（ＧＳＴ）组５３．４％～６２．１％，１１．０％～３８．６％和１４．０％～６５．０％；ｒＳｊ２８ＧＳＴ组６１．４％～６８．５％，２２．９％～６０．０％和３９．０％～７６．０％；重组日本血吸虫２３ｋｕ大亲水区表膜蛋白（ｒＳｊ２３）组５１．２％～６６．１％，２１．９％～５８．４％和５４．３％～７６．７％。以ＥＬＩＳＡ检测免疫后的绵羊血清，３类抗原均诱发高水平的特异性ＩｇＧ抗体，表明以上疫苗具有诱发绵羊产生保护性免疫的作用。Three types of vaccine candidates,recombinant paramyosin,Sj GST and Sj 23 of Schistosoma japonicum were used to immunize sheep in 1994,1995 and 1997,and marked reduction rates were obtained.The resulted mean worm reduction rates,mean reduction rates of fecal eggs,and mean reduction rates of eggs in tissues (liver,large intestine,small intestine) for n paramyosin vaccinated sheep group were 17.4%～55.3%,14.6%～68.1%,48.9%～76.7%,respectively;the corresponding reduction rates for r paramyosin group were 41.4%～44.2%,15.9%～25.1% and 41.1%～48.0%; for Sj 26 GST group, 53.4% ～62.1%,11.0%～38.6% and 14.0%～65.0%;for rSj 28 GST group,61.4%～68.5%,22.9%～60.0% and 39.09%～ 76.0%; for rSj 23 group,51.2%～66.1%,21.9%～58.4% and 54.3%～76.7%. Using ELISA detection sheep serum after immunity,high level of specific IgG was elicited by three antigens and showed that the above vaccine induced sheep to produce protective immunity.

关 键 词：日本血吸虫 重组抗原 保护性免疫 

分 类 号：S852.735[农业科学—基础兽医学]