HPLC/ESI/MS法测定卷烟烟丝和烟气中的罗汉果苷Ⅴ  

Analysis of Mogroside V in Cigarette and its Smoke Particulate Matter by HPLC/ESI/MS

作　　者：刘绍华 黄泰松 邹克兴 李桂湘 潘艳坤 孟冬玲 陈志燕 许蔼飞 韦祎 

机构地区：[1]广西中烟工业公司技术中心,广西柳州市545005 [2]广西测试分析中心,南宁市530022

出　　处：《烟草科技》2008年第7期38-41,共4页Tobacco Science & Technology

基　　金：广西壮族自治区2007年度科技攻关重点项目“烟用中草药添加剂的开发”(桂科攻0718002-2-19)

摘　　要：以Hypersil ODS C18柱(250 mm×2.1 mm i.d.,5μm)作色谱柱,柱温30℃,乙腈水混合物(体积比23∶77)为流动相,流速0.2 mL/min,采用高效液相色谱-电喷雾-质谱(HPLC/ESI/MS)法、选择离子模式(SIM)、分子离子峰m/z1285.6和四级碰撞诱导法对含罗汉果添加剂的卷烟烟丝及其烟气粒相物中的罗汉果苷V进行了定性定量分析。结果表明:①该法的回收率为100.0%,RSD≤3.5%,检出限105 ng/mL;②含罗汉果添加剂的卷烟烟丝及其烟气粒相物中的罗汉果苷V含量分别为752和248 ng/支,烟丝中罗汉果苷V向烟气中的转移率33.0%。The mogroside V in Momordica grosvenori added cigarette and its smoke particulate matter was qualitatively analyzed by four scale colliding induction mass spectrum and quantitatively determined by HPLC/ ESI/MS with Hypersil ODS C18 column （250 mm ×2.1 mm i. d. ,5 μm） at 30 ℃ and mobile phase of acetonitrile and water mixture （23： 77, v/v） at the flow rate of 0.2 mL/min under a selection ion model for molecularion （m/z1285.6）. The results indicated that： 1 ） the recovery of mogroside V was 100.0% with RSD of ≤3.5% and limit of detection of 105 ng/mL; and 2） the content of mogroside V in cigarette and its smoke particulate matter was 752 ng/cig, and 248 ng/cig. , respectively, the migration rate of mogroside V from cigarette to its smoke was 33.0%.

关 键 词：罗汉果 罗汉果苷V 高效液相色谱-电喷雾-质谱 卷烟 烟气粒相物 

分 类 号：TS411.1[农业科学—烟草工业] TS411.2