人足月胎盘间充质干细胞的分离纯化及其特征  

Isolation and biological characteristics of human term placenta mesenchymal stem cells

作　　者：陆琰[1] 陈丽[1] 张洹[1] 

机构地区：[1]暨南大学医学院血液病研究所,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2008年第4期370-374,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国务院侨办重点学科建设基金项目(51205002)

摘　　要：目的:从人足月胎盘中分离、培养间充质干细胞(MSCs),并研究其生物学特征。方法:将人足月胎盘组织经胶原酶Ⅱ消化和贴壁培养法获取间充质干细胞,运用活细胞计数和碘化丙啶(PI)检测其增殖能力;采用流式细胞术检测其细胞表面标志的表达;用地塞米松、抗坏血酸及β-磷酸甘油诱导其向成骨细胞分化,并用Von Kos-sa染色进行鉴定;用地塞米松与胰岛素诱导其向脂肪细胞分化,并以油红O染色进行鉴定。结果:从人足月胎盘分离的间充质干细胞为梭形贴壁细胞,增殖能力较强;强表达CD44、CD29,不表达CD34、CD45、CD106和HLA-DR;经诱导后向脂肪细胞及成骨细胞分化,油红O染色、Von Kossa染色为阳性。结论:人足月胎盘中也富含间充质干细胞,与其他来源的间充质干细胞的生物学特征相似,可能是组织工程新的干细胞来源。Aim： To isolate mesenehymal stem cells （MSCs） from human term placenta and study their biological characteristics. Methods： Mesenehymal stem cells were isolated from human term placenta by digestion of eollagenase Ⅱ and their unique growth characteristic of attaching to the wall of cell culture flask. The proliferation ability was detected by living cell number counting and propidium iodide staining. Their surface markers were detected by flow eytometry. The cells were induced to osteoblast with dexarnethasone, antiseorbutie acid and β-sodium glyeerophosphate. And they also were induced to adipoeytes with dexamethasone and insulin. After induction, the cells were observed by Von Kossa staining and oil red O staining. Results： Mesenchymal stem cells isolated from human term placenta were spindleshaped adherent cells. They had strong proliferation ability. They were strongly positive for CD44, CD29, but negative for CD34, CD45, CD106 and HLA-DR. After induction, oil red O staining and Von Kossa staining were positive. Conclusion：There also existed plentiful MSCs in human term placenta. They had similar biological characteristics to mesenchymal stem ceils derived from other sources. It was indicated that they might be the new stem cell source of tissue engineering.

关 键 词：胎盘 间充质干细胞 细胞培养 

分 类 号：Q813[生物学—生物工程]