T-DNA插入麝香百合的研究  

Study on the Integration of T-DNA into Lilium longiflorum

作　　者：李刚[1] 刘菊华[1] 谭光兰[1] 徐碧玉[1] 金志强[1] 

机构地区：[1]中国热带农业科学院生物技术研究所,海南海口571101

出　　处：《安徽农业科学》2008年第18期7581-7582,共2页Journal of Anhui Agricultural Sciences

基　　金：中国热带农业科学院热带生物技术研究所基本科研业务费;中国热带农业科学院基金资助项目(Rky0529)

摘　　要：[目的]获得麝香百合转基因植株。[方法]采用2步外植体法和农杆菌介导的T-DNA插入技术转化麝香百合。[结果]菌液浓度OD600值为0．6～0．8时浸染效果好，同时附加250mg／L的AS可以提高转化效率，50mg／L G418为抗性筛选最适浓度。对经过抗性筛选的百合转化植株进行PCR分子检测，部分转基因植株呈阳性，初步证明T-DNA已插入到百合基因组中。[结论]该研究为利用T-DNA插入技术筛选优良的百合新品种奠定了基础。[Objective] The aim of this study is to obtain transgenic Lilium longiflorum Thumb. [Method] A two-stop method of explant and the T-DNA integration technique were employed to transform Lilium longiflorum via Agrobacterium mediated method. [ Result ] The best infection effect appeared under the OD600 value of Agrobacterium within 0.6 - 0.8, the addition of 250 mg/L AS could increase the transformation efficiency. The optimal concentration of G418 for screening is 50 mg/L.Some putative transgenic plants of Lilium longiflorum with resistance to G418 showed positive in PCR,preliminarily proving that T-DNA gene had integrated into the genome of lily. [Conclusion] The study may lay a foundation for breeding excellent lily varieties through T-DNA integration technique.

关 键 词：麝香百合 农杆菌介导法 T-DNA 插入突变 

分 类 号：S603.6[农业科学—园艺学]