不同培养条件对牛核移植胚胎体外发育的影响  

Effect of the Different Cultivation Condiontions on Invitro Development of Bovine Embryos

作　　者：史文升[1] 村上正夫[1] 柏学进[1] 王文明[1] 刘玮[1] 董雅娟[1] 

机构地区：[1]青岛农业大学动物胚胎工程中心,山东青岛266109

出　　处：《安徽农业科学》2008年第18期7676-7677,7759,共3页Journal of Anhui Agricultural Sciences

基　　金：青岛市科技将才资助

摘　　要：[目的]进一步优化牛核移植胚胎的体外培养体系。[方法]在培养液中添加谷胱甘肽和胚胎培养的0～3d采用5％O2浓度，在不同培养条件下对牛核移植重构胚胎进行体外培养，测定卵裂率、8细胞发育率、桑椹胚率以及囊胚发育率。[结果]结果表明，试验组B的8细胞发育率（38.3％）、桑椹胚率（17．0％）和囊胚率（8.5％）与对照组A（17．9％、1．3％、1．3％）差异显著（P〈0．05）。试验组B和试验组c的桑椹胚率（17.0％、11.8％）、囊胚率（8．5％、7．8％）与对照组A（1．3％、1．3％）均差异显著（P〈0．05）。[结论]在培养液中添加谷胱甘肽和胚胎培养的0～3d采用低O2浓度（5％O2），能够提高牛核移植胚胎的体外发育率。[Objectlve]The aim of the research was to optimize the invitro development system of bovine nuclear transplant（NT） embryos, [Method]Appending the glutathione in the culture medium and reducing O2（5% ） concentration during the first 3 days, bovine nuclear transplant （NT） embryos were examined after being cultured under different conditions. Cleavage rate, 8 cell growth rate, morula rate as well as pouch embryo growth rate was determined respectively. [Result]The results indicated that 8 cell growth rate（38.3% ）, morula rate（17.0% ） and pouch embryo growth rote（8.5% ） of group B and A（17.9% 、1.3% 、1.3% ） had significant difference（ P 〈 0.05）. Morula rate（17.0%, 11.8% ） and pouch embryo growth rote（8.5%, 7.8% ） of group B and C were significantly higher （ P 〈0,05） than that of group A. [ Conclusion] Appending the glutathione that of the culture medium and reducing 02（5% 02） cencentration during the first 3 days could increase the invitro develooment rate of bovine NT embrvos,

关 键 词：牛 核移植 胚胎 体外发育 

分 类 号：S821.36[农业科学—畜牧学]