从鼠黑色素瘤细胞系中分离和鉴定癌干细胞样细胞  

Isolation and Identification of Cancer Stem Like Cells from Murine Melanoma Cell Lines

作　　者：潘猛[1,2] 文萍[1] 窦骏[1] 李雅婷[1] 唐权[1] 褚莉莉[1] 赵枫姝[1] 蒋翠莲[1] 顾宁[3] 

机构地区：[1]东南大学基础医学院病原生物学和免疫学系 [2]江苏南京210009 [3]东南大学生物科学与医学工程学院

出　　处：《生物技术通讯》2008年第4期500-502,共3页Letters in Biotechnology

基　　金：国家自然科学基金(90406023);东南大学国家自然科学基金预研项目(9223001446)

摘　　要：目的:从鼠黑色素瘤BL6F10细胞系中分离与鉴定癌干细胞(CSC)样细胞,为今后对CSC的鉴定及靶向治疗奠定基础。方法:用不同免疫磁珠标记的单克隆抗体,从BL6F10细胞系中分离有特征性CD表型的瘤细胞,体外观察不同CD表型瘤细胞在软琼脂培养基上形成克隆的能力;将这些瘤细胞皮下注射到C57BL/6小鼠,比较其致瘤性。结果:从BL6F10细胞系中分离出不同CD表型的特征性瘤细胞;在软琼脂培养基上,CD133+、CD44+和CD44+CD133+细胞克隆形成率分别高于CD133-、CD44-和CD44+CD133-细胞;CD133+、CD44+、CD44+CD133+和CD44+CD133+CD24+细胞在小鼠体内的致瘤性分别强于CD133-、CD44-、CD44+CD133-和CD44+CD133+CD24-细胞。结论:CD44+CD133+CD24+表型的BL6F10细胞的某些生物学特性与CSC样细胞相似,具有CSC特征,这些实验结果为进一步鉴定BL6F10细胞系中的CSC提供了重要的实验资料。Objective：To isolate and identify cancer stem like cells from the murine melanoma BL6F10 cell lines,and to lay a foundation for identification of cancer stem cells（CSC） and target therapy in future.Methods：The specific CD phenotype cells were isolated from the BL6F10 cells with different monoantibody labeled with immune magnetic beads by magnetic activated cell sorting system.In soft agar media,the potential clonegenesis of cells with the specific CD phenotypes were evaluated and then respectively inoculated s.c into C57BL/6 mice.After 40 days observation,the tumorigenicity of cells in mice were compared among the different cells.Results：The different CD phenotype cells were separately isolated from the BL6F10 cells.The rate of clonegenesis of CD133^＋,CD44^＋ and CD44^＋CD133^＋ cells in soft agar media were prior to CD133^-,CD44^-and CD44^＋CD133^-cells respectively.The tumorigenesis of CD133^＋,CD44^＋,CD44^＋CD133^＋ and CD44^＋CD133^＋CD24^＋ cells were superior to CD133^-,CD44^-,CD44^＋CD133^-and CD44^＋CD133^＋CD24^-cells in mice respectively.Conclusions：The CD44^＋CD133^＋CD24^＋ cells have some biological properties of cancer stem like cells,possessing characters of CSC.The investigation results provide us with an important experiment data in identifying CSC from BL6F10 cells further.

关 键 词：鼠黑色素瘤 BL6F10细胞系 癌干细胞样细胞 癌干细胞 

分 类 号：Q78[生物学—分子生物学] Q25