三七提取物含药血清对MNNG转化后GES-1细胞凋亡相关基因蛋白表达的影响(英文)  

Effects of Radix notoginseng extracts drug-containing serum on expressions of bcl-2,Bax and p21^(WAF1) proteins in MNNG transformed GES-1 cells

作　　者：李军祥[1] 王志斌[1] 朱陵群[2] 牛福玲[2] 崔巍[2] 

机构地区：[1]北京中医药大学东方医院消化内科,北京100078 [2]北京中医药大学东直门医院中医内科学重点实验室,北京100700

出　　处：《中西医结合学报》2008年第8期817-820,共4页Journal of Chinese Integrative Medicine

基　　金：教育部科学技术重点项目课题(No.2024)

摘　　要：目的:研究三七提取物犬药物血清作用于胃癌前细胞后,其凋亡相关基因蛋白表达的变化。方法:采用被N-甲基-N'-硝基-N-亚硝基胍(N-methyl-N'-nitroso-guanidine,MNNG)转化后的永生化人胃黏膜上皮细胞系GES-1细胞(简称MC细胞)作为胃癌前病变细胞的体外研究模型,用三七提取物一次性灌胃彼格犬,取给药后2 h的血清作为实验药物血清。以免疫组织化学法检测药物血清对MC细胞作用72h后bcl-2、Bax和p21WAF13种凋亡相关基因蛋白表达情况,并与正常培养的MC细胞相比较。结果:药物血清作用后的MC细胞中Bax和p21WAF1的表达较正常培养的MC细胞升高(P<0.01);Bcl-2表达较正常培养的MC细胞降低(P<0.01)。结论:三七提取物入血后可能是通过影响bcl-2、Bax和p21WAF1等对于凋亡具有调控作用的基因表达来实现对胃癌前细胞的抑制增殖及促凋亡作用的。Objective： To investigate the effects of Radix notoginseng extracts drug-containing serum on the expressions of apoptosis-regulating proteins including Bax,bcl-2 and p21^WAF1 in precancerous gastric cells.Methods： The N-methyl-N＇-nitro-N-nitroso-guanidine（MNNG） transformed eternalized human gastric mucosa epithelium GES-1 cell line（MC cell） was used in vitro as a model of gastric precancerous lesion.The medicated canine serum was prepared by feeding to the adult Beagle dog with Radix notoginseng extracts and obtaining the serum after 2-hour medication.MC cells were cultured with medicated canine serum（medicated serum group） or non-medicated canine serum（normal control group） for 72 hours.Expressions of Bax,bcl-2 and p21^WAF1 proteins were detected by immunocytochemical assay and the average optical density of the cells was determined by an image analysis system.Results： Compared with those of the normal control group,Bax and p21^WAF1 expressions in medicated serum group were significantly enhanced（P〈0.01）,while the expression of bcl-2 was significantly reduced（P〈0.01）.Conclusion： Radix notoginseng extracts may inhibit the proliferation and promote the apoptosis of precancerous gastric cells through altering expressions of the bcl-2,Bax and p21^WAF1 genes.

关 键 词：三七 胃癌 bcl-2 BAX p21-(WAF1) 

分 类 号：R285.5[医药卫生—中药学]