CTL Responses to Regulatory Proteins Tat and Rev in HIV-1 B’/C Virus-Infected Individuals  

作　　者：MING-MING JIA KUN-XUE HONG JIAN-PING CHEN HONG-WEI LIU SHA LIU XIAO-QING ZHANG HONG-JING ZHAO YI-MING SHAO 

机构地区：[1]Branch of AIDS, State Key Laboratory for Infectious Diseases Prevention and Control [2]National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100050, China

出　　处：《Biomedical and Environmental Sciences》2008年第4期314-318,共5页生物医学与环境科学（英文版）

基　　金：Ministry of Science and Technology of China (No. 2004BA719A01, 2006 CB 504207)

摘　　要：Objective To characterize HIV-1 specific CTL responses to regulatory proteins Tat and Rev in HIV-B＇/C virus-infected ART-naive individuals. Methods HIV-1-specific CTL responses were analyzed by IFN-7 ELISPOT assay using overlapping peptides spanning the consensus sequences of HIV-1 clade C Tat and Rev proteins. Statistical analysis and graphical presentation were performed using SIGMAPLOT 10.0 and SIGMASTAT 3.5. For samples with a positive response, the magnitude of CTL responses was compared between HIV-1 C proteins by Wilcoxon rank sum test, and the significance threshold was P〈0.05. Results Tat and Rev were frequently recognized, with 23% and 52% of the tested individuals having detectable responses to these proteins, respectively. Several immunodominant regions were detected in Rev. No significant correlation was observed between the magnitude and breadth of CTL responses to regulatory proteins and the control of virus replication in this study. Conclusion Tat and Rev can serve as targets for HIV-l-specific CTL, and several immunodominant regions are detectable in Rev. Further characterization of epitopes and their role in virus control may shed light on pathogenesis of HIV- 1 natural infection and also be useful for the design and testing of candidate vaccines.Objective To characterize HIV-1 specific CTL responses to regulatory proteins Tat and Rev in HIV-B＇/C virus-infected ART-naive individuals. Methods HIV-1-specific CTL responses were analyzed by IFN-7 ELISPOT assay using overlapping peptides spanning the consensus sequences of HIV-1 clade C Tat and Rev proteins. Statistical analysis and graphical presentation were performed using SIGMAPLOT 10.0 and SIGMASTAT 3.5. For samples with a positive response, the magnitude of CTL responses was compared between HIV-1 C proteins by Wilcoxon rank sum test, and the significance threshold was P〈0.05. Results Tat and Rev were frequently recognized, with 23% and 52% of the tested individuals having detectable responses to these proteins, respectively. Several immunodominant regions were detected in Rev. No significant correlation was observed between the magnitude and breadth of CTL responses to regulatory proteins and the control of virus replication in this study. Conclusion Tat and Rev can serve as targets for HIV-l-specific CTL, and several immunodominant regions are detectable in Rev. Further characterization of epitopes and their role in virus control may shed light on pathogenesis of HIV- 1 natural infection and also be useful for the design and testing of candidate vaccines.

关 键 词：HIV-1 Immune responses ELISPOT Cytotoxic T-lymphocytes 

分 类 号：R512.91[医药卫生—内科学]