牛带绦虫亚洲亚种苹果酸脱氢酶基因的生物信息学分析  

Informatics Analysis of Malate Dehydrogenase from Taenia saginata asiatica

作　　者：黄江[1] 胡旭初[2] 黄艳[2] 余新炳[2] 包怀恩[1] 郎书源[1] 廖兴江[1] 

机构地区：[1]贵阳医学院寄生虫学教研室,贵阳550004 [2]中山大学中山医学院寄生虫学教研室,广州510080

出　　处：《中国寄生虫学与寄生虫病杂志》2008年第3期225-227,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然科学基金(No.30760227);贵州省科技攻关项目(No.2006-3037)~~

摘　　要：利用美国国家生物技术信息中心(NCBI)和瑞士生物信息学研究所的蛋白分析专家系统(ExPASY)中有关基因和蛋白的序列和结构信息分析工具,结合其他生物信息学分析软件包进行分析。牛带绦虫亚洲亚种苹果酸脱氢酶(MDH)基因是全长基因,长度为1212bp,编码区为30～1028bp,编码332个氨基酸,无跨膜区,具有多个磷酸化位点,蛋白理化性质稳定;预测3个主要的抗原表位(aa95～aa100、aa322～aa327和aa117～aa122)在MDH空间结构上相距较远的分子表面,aa117～aa122属于带绦虫共有的线性抗原表位。预测胞桨型苹果酸脱氢酶是一个潜在的诊断抗原。Tools from bioinformatics websites such as NCBI, ExPaSy were used for the analysis. The malate dehydrogenase full-length gene from Taenia saginata asiatica was 1 212 bp in length, with a coding region of 30-1 028 bp and coding 332 amino acids. It was a complete and full-length gene compared with the homologues in GenBank. The protein showed no transmembrane region, with stable phycical-chemical characteristics. Three major linear epitopes located aa95-aa100, aa322-aa327 and aa117-aa122, with certain distance from each other on the surface of spatial structure of malate dehydrogenase （MDH）. The last one was the linear epitope of Taenia. This cytosolic malate dehydrogenase gene is a potential antigen for diagnosis.

关 键 词：牛带绦虫亚洲亚种 苹果酸脱氢酶 CDNA 生物信息学 

分 类 号：R383.322[医药卫生—医学寄生虫学]