载脂蛋白E基因多态性与轻-中型脑损伤急性期脑电图改变的相关性  

Correlation between polymorphism of apofipoprotein E gone and electroencephalogram after mild/moderate traumatic brain injury

作　　者：贺绪智[1] 孙晓川[1] 但炜[1] 刘福英[1] 江涌[1] 阮建[1] 

机构地区：[1]重庆医科大学附属第一医院神经外科,400016

出　　处：《中华创伤杂志》2008年第8期619-623,共5页Chinese Journal of Trauma

摘　　要：目的探讨载脂蛋白E基因（APOE）多态性与轻中型脑损伤后脑电图改变的相关性。方法（1）收集81例轻、中型脑损伤患者的临床资料，全部患者抽取静脉血2ml，采用聚合酶链反应限制性片段长度多态性（PCR—RFLP）检测APOE基因型；（2）全部患者在伤后1周内监测脑电图2～3次。APOE分型结果与脑电图及临床资料等采用SAS8．2软件分别进行x^2检验和Logistic回归分析。结果81例APOE分型分布符合遗传学Hardy—Weinberg定律；在16例APOEε4携带者中有10例（63％）脑电图加重，其比率明显高于非“携带者（25％，P=0．009）；单因素和多因素logistic回归分析均提示ε4是脑电图加重的危险因素。结论APOEε4等位基因是轻、中型脑损伤患者急性期脑电图加重的危险因素。Objective To determine the relationship between polymorphism of apolipoprotein E gene （APOE） and electroencephlogram in patients with mild/moderate traumatic brain injury. Methods （ 1 ） Venous blood for 2 ml was collected from 81 patients with mild/moderate traumatic brain injury on admission. APOE genotype was identified by PCR restriction fragment length polymorphism （ PCRRFLP）. （2） All the patients were monitored by electroencephalogram for 2-3 times within a week after injury. X^2 test and logistic regression analysis via SAS version 8.2 were performed to analyze the results of genotype and electroencephalogram and clinical data. Results The distributions of genetypes and alleles among 81 patients matched with Haldy-Weinberg Law. The findings of electroencephalogram were significantly different between patients with and without APOEe,4 （ P 〈 0.05 ）. Ten （63%） out of 16 patients with APOEe,4 showed an aggravated electroencephalogram,while only 16 （25%） out of 65 patients without APOEe,4 showed the same results of electroencephalogram. Logistic regression analyses showed that APOEe,4 was a risk factor for electroencephalogram aggravation after traumatic brain injury. Conclusion APOEe4 is a risk factor for electroencephalogram aggravation during acute stage after mild/moderate traumatic brain injury.

关 键 词：载脂蛋白E类 脑损伤 脑电图 基因多态性 

分 类 号：R686[医药卫生—骨科学]