PMA联合钙离子载体诱导K562细胞向树突状细胞分化  

Phorbol Myristate Acetate Combined with Calcium Ionophore Induces the Differentiation of K562 Cells into Dendritic Cells

作　　者：曾晓明[1] 陈君敏[1] 叶德富[1] 

机构地区：[1]福建医科大学附属第一医院血液风湿科,福州350005

出　　处：《福建医科大学学报》2008年第4期294-297,共4页Journal of Fujian Medical University

基　　金：福建省自然科学基金项目(C0210014)

摘　　要：目的探讨卟啉醇肉豆蔻酸乙酸酯(PMA)联合钙离子载体(CI)诱导K562细胞向树突状细胞(DC)分化的作用。方法对数生长期的K562细胞在含PMA和CI的培养液中培养96h后,倒置相差显微镜下观察细胞形态,用流式细胞仪检测细胞表型,用MTT法检测其刺激淋巴细胞增殖的能力。结果PMA联合CI组细胞形态发生明显变化,表面出现许多细小的突起,细胞表面CD83、CD86、CD80、CD40、HLA-DR和CD1a表达上调,并且可以刺激淋巴细胞的增殖反应。结论PMA联合CI可以有效地使K562细胞向DC分化。Objective To explore the effect of PMA （phorbol myristate acetate） and calcium ionophore （CI） in inducing the differentiation of leukemia cell line K562 into activated dendritic cells （DC）. Methods K562 cells were cultured in a common medium containing CI （A23187） and PMA for 96 h. The cell morphology was observed under the light phase contrast microscope and the electronic microscope. The viability rate of K562 cells cultured in each group was examined by the trypan blue staining. The immunologic phenotypes were analyzed by flow cytometry. The proliferation reaction of lymphocyte cells was detected by MTT colorimetry. Results After 96 h treatment with A23187 （375ug/ml） and PMA （10μg/mL）, the cells exhibited the characteristic DC morphology, CD83, CD86, CD80, CD40 and HLA-DR, CDla expression was upregulated and the cells were found to be able to strongly stimulate the proliferation of allogeneic T cells when cultured with them. Conclusion CI in combination with PMA can induce K562 cells to differentiate into DCs.

关 键 词：K562细胞 树突细胞 十四酰佛波乙酯 钙通道 离子载体 

分 类 号：R392[医药卫生—免疫学]