TGF-β蛋白在急性脊髓损伤大鼠中的研究  

Study On Expression of TGF-β in Rat’s Model with Acute Contusive Spinal Cord Injury

作　　者：周健[1] 张富国[1] 潘寒松 郭昌军[1] 

机构地区：[1]浙江省台州市第一人民医院骨科,318020

出　　处：《医学研究杂志》2008年第8期31-33,F0003,共4页Journal of Medical Research

基　　金：浙江省科技厅资助项目(2007C33026)

摘　　要：目的探讨β型转化生长因子(transforming growth factors,type beta,TGF-β)在大鼠脊髓损伤后表达的变化及其意义。方法20只SD大鼠,随机分为实验组及假手术组。实验组采用改良Allen’S打击法制作脊髓损伤动物模型,假手术组同法暴露脊髓,但不损伤脊髓。2组大鼠术后24h取手术段脊髓,用苏木精-伊红染色观察损伤脊髓组织病理变化和检测脊髓标本损伤段的TGF-β蛋白表达的差异。结果实验组脊髓HE染色显示存在大量出血坏死后形成的囊腔,组织和神经细胞水肿以及神经纤维溶解消失。免疫组化结果发现,术后第24h实验组TGF-β蛋白的表达明显增加,差别有显著性意义(P<0.01)。结论脊髓受重物打击后可上调TGF-β蛋白的表达,而这可能是脊髓损伤的机制之一。Objective To observe the expression of transforming growth factor beta（TGF-13） in rats＇ spinal cords of acute contusive injury model and try to explore the possible mechanism. Methods 20 Sprague Dawley rats were randomly divided into two groups. Ten rats underwent spinal cord contusive injury opration as the experimental group by using modified Allen＇s method （using a weight-drop device） after the T10 spinous process and the corresponding vertebral lamina were removed. The other ten rats received only T10 laminectomy without spinal cord injury as the sham-operated control group. The injured spinal cord in the two groups was taken out respectively at 24h after injury. Results Pathological alterations were detected by H-E staining and the expression of TGF-β was analyzed by immunohistochemistry analyses. From H-E staining, we could see the microstructure of spinal cord was normal in sham-operated control group and the pathological alterations were apparent in the injured spinal cord area in the experimental group as a central core lesion which consisted of spared fibers, variable cyst formations, gliosis and scarring with ＇a spare peripheral rim of tissue. We also found TGF-β expression of spinal cord was increased in experimental group when compared with sham-operated control group （P〈0.01）. Conclusion Acute contusive spinal cord injury up-regulated the expression of TGF-β,which might contribute to the pathophysiological change of spinal cord injury.

关 键 词：脊髓损伤 TGF-Β 大鼠 

分 类 号：R651.2[医药卫生—外科学] R285.5[医药卫生—临床医学]