人脐血T细胞活化的某些可视化特征  

Some Visible Properties of T Lymphocyte Activation in Human Umbilical Cord Blood

作　　者：彭新青[1] 刘华英[2] 刘永明[1] 

机构地区：[1]桂林医学院生物技术学院,541004 [2]广西师范大学生命科学学院

出　　处：《医学研究杂志》2008年第8期60-63,共4页Journal of Medical Research

基　　金：桂林医学院医药生物技术重点学科基金项目(桂教科研(2005)44号)

摘　　要：目的探讨人脐血T淋巴细胞活化的可视化特征。方法将人脐血T细胞在RPMI1640完全培养基中培养,分成两组:实验组加入抗CD3McAb(10mg/L)和适当浓度的PHA活化;对照组不加CD3McAb和PHA。测定细胞活率,倒置显微镜下观察T细胞活化的特征;制备细胞超薄切片,透射电镜下观察分析并摄影。结果活化T细胞形态良好,分裂象细胞多数不规则,胞核清晰,体积明显增大;生长旺盛的细胞区域可见到细胞集落。透射电镜下活化T细胞的染色质凝集,核仁数多而明显;典型的活化细胞中有较多空泡(特征性可视化结构)。结论人脐血T淋巴细胞活化的主要特征是细胞体积增大;染色质凝集,胞质中可出现空泡;形成细胞集落。Objective To explore visible properties of T lymphocyte activation in human umbilical cord blood. Methods T lymphocytes of human umbilical cord blood were cultured in RPMI 1640 complete medium and divided into two groups： the experimental group activated with CD3 McAb（10mg/L） plus PHA and the control group cultured without these reagents. Afcer the cell viability being measured, T cell activation was observed with inverted microscope. And then ultrathin section of the cells sample was prepared robe observed with transmission electron microscope（TEM）. Results Morphologically, activated T cells were obviously bigger, their nuclei clear, and most of divided cells had irregular outline;there were cell colonies to be seen in growing regions. Under TEM, there were more vacuoles （visible property of structure） in typically activated T lymphocytes , ehromatic agglutination and nucleoli to be observed. Conclusion Main properties of T cell activation of human umbilical cord blood were chromatic agglutination, more vacuoles in cytoplasm and cell colonies formation.

关 键 词：T淋巴细胞 活化 细胞形态学 可视化 

分 类 号：R392.12[医药卫生—免疫学] R457.7[医药卫生—基础医学]