人乳头瘤病毒2(HPV-2)变异株的突变E2蛋白转录抑制作用的研究  

Transcriptional Repressive Activity of Mutated E2 Protein of Human Papillomavirus 2(HPV-2) Variant

作　　者：雷艳君[1] 高晨[2] 姜慧英[2] 韩俊[2] 陈建明[2] 石琦[2] 周伟[2] 袁育康[1] 董小平[2] 

机构地区：[1]西安交通大学医学院,陕西西安710061 [2]中国疾病预防控制中心病毒病预防控制所,传染病预防控制国家重点实验室,北京100052

出　　处：《病毒学报》2008年第4期268-271,共4页Chinese Journal of Virology

摘　　要：皮肤寻常疣的发生与多种基因型别HPV的感染密切相关。本研究利用PCR方法对1例临床罕见的寻常疣患者感染的HPV-2毒株LCR及E2基因序列进行扩增、测序,分别构建含HPV-2变异株及原毒株LCR的重组CAT基因报导质粒pBLCAT-LCR和表达突变及野生型E2蛋白的重组真核表达质粒pcDNA3.1-E2,通过瞬时转染HeLa细胞,研究变异株启动子活性及突变E2蛋白的转录抑制作用。结果显示,患者感染的HPV-2变异株LCR及E2基因均存在多处点突变。变异株早期启动子活性明显高于原毒株;突变的E2蛋白转录抑制作用较野生型E2蛋白显著降低;变异株LCR上E2结合位点核苷酸的突变明显降低E2蛋白对病毒早期启动子的抑制作用。提示HPV-2变异株启动子活性增强及突变E2蛋白转录抑制作用的降低与这一罕见巨大寻常疣临床表型之间存在着重要的联系。Common warts are close associated with HPVs infection. In this study, we amplified and sequenced the LCR fragment and E2 gene of HPV-2 that infected the patient of extensive common wart with cutaneous horns, and we constructed the recombinant CAT-reporter plasmids pBLCAT-LCR containing HPV-2 prototype or variant LCR and mammalian expression plasmids pcDNA3. 1-E2 containing prototype or variant E2 ORF individually. The promoter activities of HPV-2 variant and the transcriptional repression activities of the mutated E2 protein were evaluated by transient transfection into HeLa cells. The results showed that there were several mutations in LCR and E2 gene of HPV-2 variant. Compared with the prototype, the viral early promoter activity of variant was significantly increased uder the control of LCR. Compared with the wild type E2 protein, the transcriptional repression activities of the mutated E2 protein was abolished partially. We speculate herein that increased promoter activities and decreased repression effect of the mutated E2 protein are linked, at least partially, with the clinical phenotypes of the uncommon huge common wart.

关 键 词：HPV-2 寻常疣 E2 LCR 启动子活性 

分 类 号：R373.9[医药卫生—病原生物学]