脂筏在人类疱疹病毒6型装配中的作用  

Role of Lipid Raft in Assembly of Human Herpesvirus 6

作　　者：黄红兰[1] 李凡[1] 

机构地区：[1]吉林大学白求恩医学院病原生物学教研室,吉林长春130021

出　　处：《病毒学报》2008年第4期295-299,共5页Chinese Journal of Virology

基　　金：卫生部笹川医学奖学金同学会科研启动基金资助项目(093)

摘　　要：为了探讨脂筏在人类疱疹病毒6型(HHV-6)装配中的作用,用HHV-6 GS株感染HSB2细胞,用非离子去污剂Triton X-100提取脂筏成分,利用Western blot分析HHV-6包膜糖蛋白与脂筏的相关性。并用免疫荧光双标记的方法,从分子共定位的角度研究HHV-6糖蛋白B(gB)与GPI(glycosyl-phosphatidyl inosital)锚固蛋白CD59分子以及神经节苷脂GM1(monosialotetrahexosyl ganglioside)分子之间的表达与分布关系。结果发现HHV-6包膜糖蛋白B、H、L、Q1和Q2(gB、gH、gL、gQ1和gQ2)分布在脂筏部位。激光共聚焦显微镜可观察到CD59分子及GM1均与HHV-6包膜糖蛋白B有着相同的分布,即脂筏提供HHV-6装配的平台。关于脂筏在人类疱疹病毒6型装配中的作用,这是第一次报道。To explore the role of lipid raft in assembly of human herpesvirus 6, the HHV-6 GS strain was applied to infect the HSB2 cells and then the lipid raft composition was extracted from the cells with nonionic detergent Triton-X 100. The relationship between the HHV-6 envelope glycoprotein and lipid raft was analyzed by Western Blot. Immunofluorescence double-staining was used to study the colocalization of the HHV-6 glycoprotein B（gB） with GPI anchored protein CD59 and ganglioside GM respectively. HHV- 6 envelope glycoprotein B,H,L,Q1 and Q2 （gB,gH,gL,gQ1 and gQ2 ） were all existed in the lipid raft. Moreover, CD59 and HHV-6 envelope glycoprotein B showed the same localization through the confocal microscope. We concluded the lipid raft provided the platform for HHV-6 assembly. This is the first report concerning to the role of lipid raft in assembly of human Herpesvirus 6.

关 键 词：人类疱疹病毒6型 脂筏 装配 

分 类 号：R373.2[医药卫生—病原生物学]