表达HPV16 L1抗原的1型重组AAV载体免疫效果的研究  

Immune Response Induced by Vaccination with Pseudotyped rAAV1 Expressing HPV16 L1 Protein

作　　者：周玉柏[1] 李泽琳[1] 周玲[2] 盛望[1] 马洪涛[1] 曾毅[1] 

机构地区：[1]北京工业大学生命科学与生物工程学院病毒药理室,北京100022 [2]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室,北京100052

出　　处：《病毒学报》2008年第4期300-304,共5页Chinese Journal of Virology

基　　金：北京市科委资助项目(D0906008000091)

摘　　要：为研究1型重组腺病毒伴随病毒(Adeno-associated virus type 1,AAV1)载体作为HPV16预防性疫苗的可行性,构建含密码子优化型HPV16L1基因(mod.HPV16L1)的1型重组AAV载体rAAV1-mod.HPV16L1,将纯化的rAAV1-mod.HPV16L1以肌注和滴鼻途径分别免疫C57BL/6小鼠,使用体外中和实验检测血清中的特异性中和抗体。结果显示,rAAV1-mod.HPV16L1单针肌注及滴鼻免疫均可诱导特异性血清中和抗体,但二组抗体动态变化趋势不同,肌注组血清中和抗体滴度显著高于滴鼻组。rAAV1-mod.HPV16L1单针肌注免疫可诱导强而持久的血清中和抗体,是理想的候选HPV16预防性疫苗。To investigate the feasibility of using recombinant adeno-associated virus type 1 vector as prophylactic vaccine against HPV16 infection, rAAV1-mod. HPV16L1, the recombinant AAV1 vector containing codon-modified HPV16 L1 gene, was constructed. C57BL/6 mice were immunized with purified rAAV1 vector through intramuscular and intranasal inoculation routes, and the titer of neutralizing antibody was determined by neutralization assay based on HPV16 pseudovirus. The result shows that the single dose of rAAV1-mod. HPV16L1 can induce specific neutralizing antibody in serum through both inoculation routes. Compared with intranasal group, intramuscular group can induce higher titer of neutralizing antibody. Eliciting strong and prolonged neutralizing antibody in serum, the rAAV1-mod. HPV16L1 is one of promising HPV16 prophylactic vaccine candidates.

关 键 词：乳头瘤病毒 人 基因 L1 腺病毒伴随病毒 

分 类 号：R373.9[医药卫生—病原生物学]