人免疫缺陷病毒对不同黏膜上皮细胞系的感染能力的研究  

Infectivity of different human immunodeficiency virus strains for mucosal epithelial cell lines

作　　者：李悦[1,2] 赵辉[1] 杜军[1] 全宇[1] 邢辉[1] 陈启民[2] 邵一鸣 杨贵波[1] 

机构地区：[1]中国疾病预防控制中心性病艾滋病预防控制中心传染病预防控制国家重点实验室,北京100050 [2]南开大学生命科学学院

出　　处：《中华微生物学和免疫学杂志》2008年第7期577-581,共5页Chinese Journal of Microbiology and Immunology

基　　金：基金项目：国家自然科学基金资助项目（30571750）;国家科技部973资助项目（2005CB522903）

摘　　要：目的研究人免疫缺陷病毒（HIV-1）对不同黏膜上皮细胞系的感染能力。方法用实验室株HIV-1 SF33和2株原代HIV-1（02010561，02010141）分别感染Caco-2、T-84和HeLa3株黏膜上皮细胞和MT-4细胞。接种病毒后间隔3～4d采集培养上清检测P24并用实时定量RT-PCR检测病毒载量；采集细胞提取DNA并用PCR法检测感染细胞中病毒DNA和整合入细胞基因组内的病毒DNA。结果所用3株病毒都可以产毒性地感染阳性对照细胞MT-4，对整合病毒DNA的PCR检测发现它们均能够整合到MT-4细胞基因组内；实验室适应株HIV-1 SF33虽然能够感染所有3株上皮细胞，但它不能整合入Caco-2细胞的基因组中；虽然2株原代分离病毒均能感染T-84细胞，但只有HIV-1 02020141能够整合入T-84细胞的基因组中，原代分离病毒HIV-1 02010561能够感染HeLa细胞，但不能整合到其基因组中。结论虽然HIV-1的实验室毒株和原代分离毒株都可能感染黏膜上皮细胞，但它们在黏膜上皮细胞中建立稳定产毒性感染（感染并产生病毒）的能力因细胞和毒株不同而异。Objective To compare the infectivity between laboratory adapted human immunodefi- ciency virus（HIV-1） and primary HIV-1 isolates for different mucosal epithelial cell lines. Methods Mu- cosal epithelial cells Caco-2, T-84, HeLa and lymphocyte MT-4 were infected with laboratory adapted HIV-1 SF33 and 2 primary HIV-1 isolates （02010561, 02010141）. Culture supernatant and cells were collected respectively on 3-4 days interval after virus inoculation. The former was tested for HIV-1 antigen P24 level and viral load, and the latter was tested for total viral DNA and integrated viral DNA. Results All 3 virus strains could infect MT-4 cells and integrate into their genome. Only HIV-1 SF33 could infect Caco-2 cells but could not integrate into their genomic DNA. Both HIV-1 SF33 and 02010561 infected HeLa cells but only integration of HIV-1 SF33 was detected. All the 3 HIV-1 strains infected T-84 cells but only the integra- tion of HIV-1 SF33 and 02010141 was observed. Conclusion Although laboratory adapted and primary HIV-1 strains are able to infect human mucosal epithelial cell lines, transient or productive infection established in different mucosal epithelial cells is dependent on the character of cells and virus strains.

关 键 词：人免疫缺陷病毒 黏膜上皮细胞 感染 整合 

分 类 号：R3[医药卫生—基础医学]