热休克蛋白90与戊型肝炎病毒重组衣壳蛋白P239相互作用的初步研究  

Studies on the interaction between HSP90 and hepatitis E virus recombinant particle P239

作　　者：郑子峥[1] 苗季[1] 吴小成[1] 何水珍[1] 唐明[1] 孙媛媛[1] 王颖彬[1] 杜海莲[1] 张军[1] 夏宁邵[1] 

机构地区：[1]厦门大学国家传染病诊断试剂与工程疫苗技术研究中心,361005

出　　处：《中华微生物学和免疫学杂志》2008年第7期582-586,共5页Chinese Journal of Microbiology and Immunology

基　　金：基金项目：福建省青年科技人才创新项目（2006F3124）;福建省科技重大专项（2004YZ01）

摘　　要：目的对前期研究中所筛选出的能够与戊型肝炎病毒（hepatitis E virus，HEV）重组衣壳蛋白颗粒P239存在特异性的相互作用的蛋白进行分析验证。方法使用pull-down、MALDI-TOF-MS、免疫共沉淀技术及免疫荧光技术对与P239有潜在相互作用的蛋白进行鉴定和进一步分析。结果MALDI-TOF-MS及免疫共沉淀技术，均表明该蛋白为热休克蛋白90（HSP90）并与P239相互作用。随后，对P239进入细胞的过程中胞内HSP90蛋白量及其分布的变化做了初步研究。发现虽然HSP90在蛋白总量上并没有发生明显的变化，但其定位却伴随着P239在胞内的迁移发生了由细胞膜向细胞核核周的转移。结论HSP90可能参与了P239入胞后的转运并介导了HEV入胞后向效应细胞器的转运，为研究HEV的感染机制及对HEV的预防和控制提供了有益的线索。Objective To identify the protein interacting with hepatitis E virus （HEV） recombinant capsomeric particles（P239）. Methods Protein interacting with HEV was analyzed by the pull-down, MALDI-TOF-MS, co-immunoprecipitation （Co-IP） and CONFOCAL. Results A protein interacting with HEV recombinant particle （P239） was identified as HSP90 by MALDI-TOF-MS. The interaction between HSP90 and P239 was further confirmed by Co-IP. The protein level and localization of HSP90 and P239 in HepG2 were detected. The total quantity of HSP90 didn＇t change, and the movement of HSP90 from plasma membrane to perinuclei region with P239 was observed. Conclusion HSP90 may play an important role in the trafficking of P239. It suggests that HSP90 participate in the transportation of HEV after infection, which may contribute to the prevention and control of the disease.

关 键 词：戊型肝炎病毒 热休克蛋白90 蛋白相互作用 

分 类 号：R3[医药卫生—基础医学]