重建肝动脉血供的小鼠原位肝移植模型的建立  

The establishment of an arterialized liver transplantation model in mice

作　　者：张玉君[1] 陈耿[1] 李晓武[1] 杨程[1] 王曙光[1] 别平[1] 李昆[1] 董家鸿[2] 

机构地区：[1]重庆第三军医大学全军肝胆外科研究所,400038 [2]北京解放军总医院外科

出　　处：《中华普通外科杂志》2008年第7期503-506,共4页Chinese Journal of General Surgery

基　　金：国家自然科学基金（30571834）;全军医药卫生科研基金资助（06G071）.志谢 衷心感谢本所临床研究中心马凤溪老师为本文绘制了精美的插图.

摘　　要：目的构建稳定的重建肝动脉血供的小鼠原位肝移植模型，初步探讨重建肝动脉血供在小鼠肝移植中的意义。方法66只小鼠分为实验组（n=33）和对照组（n=33）。实验组行重建肝动脉血供的原位肝移植术，在经典“双袖套法”的基础上，采用支架法重建肝动脉血供；对照组行非动脉化的原位肝移植。结果共施行小鼠原位肝移植共66只。术后2周实验组生存率为84．85％（28／33），对照组生存率为81．82％（27／33）；实验组的血清γ-GT和ALP水平显著低于对照组；病理学证据亦提示实验组胆管上皮细胞受损较对照组为轻。结论肝动脉血供重建有助于减轻移植肝胆道冷保存．再灌注损伤。该模型可能成为肝移植分子生物学研究的理想动物模型。Objective To establish a stable orthotopic liver transplantation （OLT）model with hepatic artery reconstruction in mice. Methods Of the 66 OLT mice, 33 recipient mice underwent conventional ＇two cuff＇ technique without hepatic artery reconstruction （control group ）, 33 with hepatic artery reconstruction using ＇ stent＇ technology （experimental group）. Results The 2 week posttransplant survival rate was 84.85% （28/33） and 81.82% （27/33） in the arterialized and nonarterialized groups respectively. Serum γ-GT and ALP level of arterialized recipient were much lower than those of nonarterialized animals. The pathology of the donor liver showed that rearterialization helps to relieve bile duct injury of liver allograft, Conclusions The hepatic artery supply plays a central role in preventing bile duct injury after transplantation. This mouse OLT model may meet the need for the study of problems associated with liver transplantation.

关 键 词：肝脏移植 动物实验 血管重建 

分 类 号：R686[医药卫生—骨科学]