纤维连接蛋白及精-甘-天冬-丝氨酸多肽对肝癌细胞化疗敏感性的影响  

The effect of fibronectin and arginine-glycine-aspartic-serine tetrepeptide on chemotherapy sensitivity of fiver carcinoma cell fines

作　　者：吴忠明[1] 王伟林[1] 金晶[1] 谢海洋[1] 周琳[1] 郑树森[1] 

机构地区：[1]浙江大学医学院附属第一医院肝胆胰外科、卫生部多器官联合移植研究重点实验,杭州310003

出　　处：《中华普通外科杂志》2008年第7期537-539,共3页Chinese Journal of General Surgery

摘　　要：目的探讨纤维连接蛋白（FN）和精-甘-天冬-丝氨酸多肽（RGDS多肽）对肝癌细胞化疗敏感性的影响。方法将肝癌细胞株7721分为胎牛血清BSA处理组（BO组）、纤维连接蛋白FN处理组（FO组）、精-甘-天冬-丝氨酸RGDS多肽处理组（RO组）、FN＋RGDS多肽共同处理组（FR组），加入不同浓度的丝裂霉素C（MMC）作用2h、12h和24h，用MTT比色法检测不同时间肿瘤细胞的存活率，用荧光染色法和流式细胞术检测肿瘤细胞凋亡，比较FN和RGDS对化疗作用结果的影响。结果不同浓度MMC作用2h后，FO组及BO组、FO组与FR组之间的存活率比较，差异无统计学意义（P〉0．05），而作用12h、24h后，FO组及BO组、FO组与FR组之间的存活率比较，差异有统计学意义（P〈0．05）。结论药物作用一定时间后，FN能使MMC对肝癌细胞的促凋亡作用下降，产生药物耐受；而RGDS多肽上调肿瘤细胞对化疗药物的敏感性。Objective To study the effect of fibronectin （FN） and arginine-glycine-aspartate- serine tetrapeptide （RGDS） on chemotherapy sensitivity of hepatocellular carcinoma （HCC） cell lines. Methods A HCC cell lines 7721 was divided into BSA group（ BO）, FN group（ FO）, RGDS group（RO） and FN ＋ RGDS group（FR）. Mitomycin-C（MMC） was added into each group with different concentrations for 2 h, 12 h and 24 h. MTr assay was used to detect the survival rate of the tumor cell at different time, and fluorescent staining method and flow cytometry was used to detect the tumor cell＇s apoptosis. Results The survival rate and apoptosis rate was significantly different between each group with the treatment of MMC for 12 h or 24 h, and no statistical difference was found after incubation for 2 h. Conclusions With time FN down-regulates MMC induced apoptosis of the HCC cells, making HCC cells less sensitive to chemotherapy, while RGDS enhances the sensitivity of HCC cells to chemotherapy.

关 键 词：癌 肝细胞 纤维蛋白 精-甘-天冬-丝氨酸多肽 

分 类 号：R686[医药卫生—骨科学]