Comparative analysis of mitochondrial fragments transferred to the nucleus in vertebrate  

作　　者：Hongying Qu Fei Ma Qingwei Li 

机构地区：[1]Institute of Marine Genomics ＆ Proteomics, Liaoning Normal University, Dalian 116029, China

出　　处：《Journal of Genetics and Genomics》2008年第8期485-490,共6页遗传学报（英文版）

基　　金：the National Natural Science Foundation of China (No. 30470936)

摘　　要：Mitochondrial DNA sequences transferred to the nucleus give rise to the so-called nuclear mitochondrial DNA （numt）. In the GenBank database, 244 numts have been found in six orders of birds （Anseriformes, Columbiformes, Falconiformes, Charadriiformes, Galliformes and Passeriformes）. Sequences alignment （NCBI-BLASTN） was carded out with mitochondrial and corresponding nuclear genome sequences in nine vertebrate species. The sequences with high homology were considered as numts. The number of numts ranged from 15 in chicken to 159 in chimpanzee. The sequences of numts in macaque, chimpanzee, and human spanned 100% of the entire mammalian mitochondrial genome. The reconstructed frequency of the mitochondrial gene transferred to the nucleus demonstrated that the rRNA genes had high frequencies than other mitochondrial genes. Using the RepeatMasker program, the transposable elements were detected in the flanking regions of each numt. The results showed that less than 5% of the flanking sequences were made up of repetitive elements in chicken. The GC content of 5＇- and 3＇-flanking regions of numts in nine species was less than 44%. The analysis of the flanking sequences provided a valuable understanding for future study on mechanism of mitochondrial gene transfer to the nucleus and the site of numt integration.Mitochondrial DNA sequences transferred to the nucleus give rise to the so-called nuclear mitochondrial DNA （numt）. In the GenBank database, 244 numts have been found in six orders of birds （Anseriformes, Columbiformes, Falconiformes, Charadriiformes, Galliformes and Passeriformes）. Sequences alignment （NCBI-BLASTN） was carded out with mitochondrial and corresponding nuclear genome sequences in nine vertebrate species. The sequences with high homology were considered as numts. The number of numts ranged from 15 in chicken to 159 in chimpanzee. The sequences of numts in macaque, chimpanzee, and human spanned 100% of the entire mammalian mitochondrial genome. The reconstructed frequency of the mitochondrial gene transferred to the nucleus demonstrated that the rRNA genes had high frequencies than other mitochondrial genes. Using the RepeatMasker program, the transposable elements were detected in the flanking regions of each numt. The results showed that less than 5% of the flanking sequences were made up of repetitive elements in chicken. The GC content of 5＇- and 3＇-flanking regions of numts in nine species was less than 44%. The analysis of the flanking sequences provided a valuable understanding for future study on mechanism of mitochondrial gene transfer to the nucleus and the site of numt integration.

关 键 词：mitochondrial DNA NUMTS nuclear insertion flanking sequence VERTEBRATE 

分 类 号：Q959.3[生物学—动物学] S839.1[农业科学—畜牧学]