毛细管电泳-激光诱导荧光分离检测手性5-(2-氨基丙基)-2-甲氧基苯磺酰胺对映体  

Capillary electrophoresis for chiral separation and laser-induced fluorescence detection of 5-(2-aminopropyl)-2-methoxybenzenesulfonamide enantiomers

作　　者：吴东兵[1] 高原[2] 吴振刚[3] 李华[2] 

机构地区：[1]延安大学化学与化工学院无机化学教研室,716000 [2]西北大学分析科学研究所中药现代化研究中心 [3]华北煤炭医学院药学系

出　　处：《中国药物与临床》2008年第8期597-599,共3页Chinese Remedies & Clinics

基　　金：国家自然科学基金资助项目(20675063);高校博士点专项科研基金资助项目(20060697009)

摘　　要：目的建立毛细管电泳——激光诱导荧光法分离测定(R/S)-5-(2-氨基丙基)-2-甲氧基苯磺酰胺对映体。方法用异硫氰酸荧光素衍生(R/S)-5-(2-氨基丙基)-2-甲氧基苯磺酰胺,衍生产物在70mmol/L的硼砂(pH=9.5)缓冲液中用毛细管电泳分离,激光诱导荧光方法测定。结果(R/S)-5-(2-氨基丙基)-2-甲氧基苯磺酰胺对映体得到了基线分离,线性范围均为8.20×10-8～9.02×10-7mol/L。结论本方法简单,精密度高,灵敏度高,可为盐酸坦索罗辛的质量控制提供方法。Objective To establish an HPCE-LIF method for separation and determination of the intermediate of tamsulosin hydrochloride, （R/S）-5-（2-aminopropyl）-2-methoxybenzenesulfonamide （R/S-APMB）. Methods （R）-5- （2-aminopropyl）-2-methoxybenzenesulfonamide reacted with fluorescein isothiocyanate （FITC） and then FITC-R-APMB was obtained. FITC-R-APMB was separated using 70 mmol/L borate as a buffer （pH=7.5）. The 47 cm uncoated fused-silica capillary with 75 μm internal diameter （effective length 40 cm） was used. The capillary temperature was maintained at 20 ℃. Samples were injected at a pressure of 3.45 kPa in 1 s and separated at 20 kV. Results Both R- and S-APMB had low detection limits （8.2×10^-9 mol/L）. The linear range of R- APMB was 8.20×10^-8-9.02×10^-7 mol/L. Conclusion The HPCE-LIF method may offer a simple and highly sensitive approach for quality control of tamsulosin production.

关 键 词：电泳 毛细管 激光诱导荧光 手性分离 盐酸坦索罗辛 中间体 荧光素 

分 类 号：R927[医药卫生—药学]