LDL对绵羊精子冷冻保存效果的研究  

Effects of LDL on Cryopreservation of Sheep Spermatozoa

作　　者：宏伟[1] 倪利平[1] 田东海[1] 芦永跃[1] 邓常胜[1] 李金泉[1] 张小宇[1] 郑云胜[1] 

机构地区：[1]内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特010018

出　　处：《畜牧与饲料科学》2008年第4期29-31,共3页Animal Husbandry and Feed Science

摘　　要：绵羊冷冻精液的受精率低于自然交配,其原因之一可能是在冷冻过程中精子质膜损伤严重造成的。该研究以内蒙古细毛羊为试验动物,在稀释液中以低密度脂蛋白(LDL)代替卵黄。分析了冷冻过程中对精子活率、顶体完整率和质膜完整率的影响。筛选出了最优的LDL稀释液配方,以提高绵羊精液质量,减少精子的损伤,为提高绵羊精子冷冻保存后的受精率提供理论依据。The fertility test showed that pregnant rate of AI with frozen semen were significantly lower than that of natural mating. One of the reasons might be the sperm plasma membrane was serious injured during freezing. Therefore, the paper studied on sheep spermatozoa during freezing which was protected with egg yolk （EY） and local fine wool sheep was used as the experimental animals. Then low density lipoprotein （LDL） was used in diluents instead of egg yolk to study on the effect of LDL on sperm motility, acrosome intact rate and plasma membrane integrity. The optimal LDL dilution formula was screened to improve sheep semen quality, reduce the damage sperm, and provide theoretical basis for improving fertility of ram sperm after cryopreservation.

关 键 词：绵羊精液 低密度脂蛋白(LDL) 卵黄 冷冻保存 精子质量 

分 类 号：S826.34[农业科学—畜牧学]