NOS在子宫内膜异位症患者在位内膜和异位内膜中的表达  

Nitric oxide synthase expression in eutopic and ectopic endometrial of women with endometriosis

作　　者：涂灵[1] 邬欢欢[1] 

机构地区：[1]江西省妇幼保健院生殖健康科,330006

出　　处：《中国妇幼保健》2008年第23期3308-3310,共3页Maternal and Child Health Care of China

摘　　要：目的:探讨一氧化氮合酶(NOS)在子宫内膜异位症患者在位内膜和异位内膜中的表达。方法:采用逆转录聚合酶链式反应(RT-PCR)方法对20例正常子宫内膜和30例子宫内膜异位症患者在位和异位内膜进行内皮型NOS(eNOS)和诱导型NOS(iNOS)表达的半定量检测。结果:eNOS在子宫内膜的增生期和分泌期均有表达,增生期在位内膜为2.47±0.38,异位内膜为2.53±0.51,对照组为1.73±0.50,分泌期在位内膜为3.37±0.41,异位内膜为3.43±0.44,对照组为3.16±0.55,增生期eNOS的表达均高于对照组(P=0.01),分泌期eNOS的表达无统计学意义(P=0.064)。iNOS只在子宫内膜的分泌期有微量表达,在位内膜为1.17±0.22,异位内膜为1.33±0.28,对照组为0.86±0.24,分泌期iNOS的表达与对照组相比有统计学意义(P=0.016)。在位内膜和异位内膜中eNOS和iNOS的表达在月经各周期相比较均无统汁学意义(P值分别为0.25、0.106、0.073)。结论:NOS在子宫内膜异位症患者子宫内膜上的高表达可能与其发病机制存在一定的关系。Objective： To explore the expression of endothelial and inducible nitric oxide synthase （eNOS and iNOS） in eutopic and ectopic endometrial of women with endometriosis. Methods： Reverse transcriptase polymerase chain reaction （RT - PCR） was performed on endometriosis tissues obtained from controls （n = 20） and on eutopic/ectopic endometriosis tissues from endometriosis patients （n = 30） to evaluate eNOS and iNOS concentrations in these endometriosis tissues. Results： eNOS were expressed in both endometrial hyperplasia period and secretion period. In endometrial hyperplasia period in eutopic/ ectopic endometriosis tissues , the eNOS concentrations were 2. 47 ± 38/2.53 ±51, and it was 1.73 ± 50 in control group; In the secretion period in eutopic / ectopic endometriosis tissues, the eNOS concentrations were 3.37 ± 4/3.43 ± 44, and it was 3. 16 ± 55 in control group. The expression of eNOS in endometrial hyperplasia period were higher in the endometrial tissues of women with endometriosis than that in control group （ P = 0. 01 ）, the expression of eNOS was no significant in endometrial hyperplasia period, iNOS only had a micro - expression in secretion period, in eutopic/ectopic endo- metriosis tissues , the eNOS concentrations were 1.17 ± 22/1.33 ±0. 28, it was 0. 86 ± 24. in control group. Endometrlosis appeared to contain more iNOS than those of controls in secretion period （ P = 0. 016 ） . The expression of eNOS and iNOS in eutopic/ectopic endometri- osis tissues had no significance in different stage during the menstrual cycle compared with the EC juice （ P values were 0. 25, 0. 106, 0. 073） . Conclusion： Greater amounts of NOS are present in the endometrial tissues of women with endometriosis, implying a possible role for NOS in the pathogenesis of endometriosis.

关 键 词：子宫内膜异位症 一氧化氮合酶 内皮型NOS 诱导型NOS 逆转录聚合酶链式反应 

分 类 号：R711.71[医药卫生—妇产科学]